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Western Blot (WB)

Rabbit anti-All species Glutathione S-transferases, GST Polyclonal Antibody | anti-GST antibody

Anti-Glutathione S-transferases, GST antibody

Reactivity
All species
Applications
Western Blot, Immunoprecipitation
Purity
Immunogen affinity purified
Synonyms
Glutathione S-transferases; GST; Polyclonal Antibody; Anti-Glutathione S-transferases; GST antibody; Polyclonal Anti- Glutathione S-transferases; anti-GST antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
All species
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified
Form/Format
Liquid
Applicable Applications for anti-GST antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB (1:10,000-100,000); ELISA (1:10,000); IP (1:50-200)
Immunogen
Recombinant GST protein expressed by pGEX.
Storage Buffer
In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
Storage Buffer
In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
Preparation and Storage
Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles

Western Blot (WB)

Western Blot (WB)
Related Product Information for anti-GST antibody
Glutathione S-transferases are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GST is commonly used to create fusion proteins. The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the DYKDDDDK-tag, is quite big. Genetic polymorphisms in glutathione S-transferase and its altered expression and activity are associated with oxidative DNA damage and also damage of the kidney. Subsequently the individual's risk of cancer susceptibility increases.1
References
1. Khan MA, Tania M, Zhang D, Chen H (2010). "Antioxidant enzymes and cancer". Chin J Cancer Res 22 (2): 87-92.

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Product Notes

The GST (Catalog #AAA355205) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Glutathione S-transferases, GST antibody reacts with All species and may cross-react with other species as described in the data sheet. AAA Biotech's Glutathione S-transferases, GST can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB (1:10,000-100,000); ELISA (1:10,000); IP (1:50-200). Researchers should empirically determine the suitability of the GST for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutathione S-transferases, GST, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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