Rabbit anti-Human, Mouse SEMA6D Polyclonal Antibody | anti-SEMA6D antibody

SEMA6D, NT (SEMA6D, KIAA1479, Semaphorin-6D)

Cat. Num. AAA649629

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: SEMA6D; Polyclonal Antibody; NT (SEMA6D; KIAA1479; Semaphorin-6D); Anti -SEMA6D; anti-SEMA6D antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-SEMA6D antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: SEMA6D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-48 amino acids from the N-terminal region of human SEMA6D.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse cerebellum tissue lysates (35ug/lane) using MBS649629. This demonstrates that MBS649629 detected the SEMA6D protein (arrow).)

Related Product Information for anti-SEMA6D antibody

Semaphorins are a large family, including both secreted and membrane associated proteins, many of which have been implicated as inhibitors or chemorepellents in axon pathfinding, fasciculation and branching, and target selection. All semaphorins possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Additional sequence motifs C-terminal to the semaphorin domain allow classification into distinct subfamilies. Results demonstrate that transmembrane semaphorins, like the secreted ones, can act as repulsive axon guidance cues. This gene encodes a class 6 vertebrate transmembrane semaphorin that demonstrates alternative splicing. Several transcript variants have been identified and expression of the distinct encoded isoforms is thought to be regulated in a tissue- and development-dependent manner. [provided by RefSeq].

Product Categories/Family for anti-SEMA6D antibody

Antibodies; Abs to Plexin; Semaphorin

NCBI and Uniprot Product Information

• NCBI GI #: 312222667
• NCBI GeneID: 80031
• NCBI Accession #: NP_001185928.1
• NCBI GenBank Nucleotide #: NM_001198999.1
• UniProt Accession #: Q8NFY4; Q8NFY3; Q8NFY5; Q8NFY6; Q8NFY7; Q9P249; A6NF10; A6NNK1; A7E2A0
• Molecular Weight: 119,872 Da
• NCBI Official Full Name: semaphorin-6D isoform 1
• NCBI Official Synonym Full Names: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
• NCBI Official Symbol: SEMA6D
• NCBI Protein Information: semaphorin-6D
• UniProt Protein Name: Semaphorin-6D
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA6D
• UniProt Synonym Gene Names: KIAA1479
• UniProt Entry Name: SEM6D_HUMAN

NCBI Description

Semaphorins are a large family, including both secreted and membrane associated proteins, many of which have been implicated as inhibitors or chemorepellents in axon pathfinding, fasciculation and branching, and target selection. All semaphorins possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Additional sequence motifs C-terminal to the semaphorin domain allow classification into distinct subfamilies. Results demonstrate that transmembrane semaphorins, like the secreted ones, can act as repulsive axon guidance cues. This gene encodes a class 6 vertebrate transmembrane semaphorin that demonstrates alternative splicing. Several transcript variants have been identified and expression of the distinct encoded isoforms is thought to be regulated in a tissue- and development-dependent manner. [provided by RefSeq, Nov 2010]

Uniprot Description

Function: Shows growth cone collapsing activity on dorsal root ganglion (DRG) neurons in vitro. May be a stop signal for the DRG neurons in their target areas, and possibly also for other neurons. May also be involved in the maintenance and remodeling of neuronal connections.

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein Ref.1. Isoform 2: Cell membrane; Single-pass type I membrane protein Ref.1. Isoform 3: Cell membrane; Single-pass type I membrane protein Ref.1. Isoform 4: Cell membrane; Single-pass type I membrane protein Ref.1. Isoform 5: Cell membrane; Single-pass type I membrane protein Ref.1. Isoform 7: Cytoplasm Ref.1.

Sequence similarities: Belongs to the semaphorin family.Contains 1 PSI domain.Contains 1 Sema domain.

Sequence caution: The sequence BAA96003.2 differs from that shown. Reason: Erroneous initiation.