Rabbit anti-Human MKRN1 Polyclonal Antibody | anti-MKRN1 antibody

MKRN1, ID (MKRN1, RNF61, E3 ubiquitin-protein ligase makorin-1, RING finger protein 61) (Azide free) (HRP)

Cat. Num. AAA6321002

• Gene Names: MKRN1; RNF61
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MKRN1; Polyclonal Antibody; ID (MKRN1; RNF61; E3 ubiquitin-protein ligase makorin-1; RING finger protein 61) (Azide free) (HRP); RING finger protein 61; anti-MKRN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MKRN1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MKRN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-213 amino acids from the Central region of human MKRN1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MKRN1 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the MKRN1 antibody detected the MKRN1 protein (arrow).)

Related Product Information for anti-MKRN1 antibody

The Makorin ring finger protein-1 gene (MKRN1) is a highly transcribed, intron-containing source for a family of intronless mammalian genes encoding a novel class of zinc finger proteins. Phylogenetic analyses indicate that the MKRN1 gene is the ancestral founder of this gene family (Gray et al., 2000 [PubMed 10843807]).

Product Categories/Family for anti-MKRN1 antibody

Antibodies; Ubiquitin Ligases

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Ko, A., et al. J. Virol. 84(1):426-436(2010). Lee, E.W., et al. EMBO J. 28(14):2100-2113(2009). Shimada, H., et al. BMC Cancer 9, 232 (2009): Omwancha, J., et al. Endocrine 29(2):363-373(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 223468620
• NCBI GeneID: 23608
• NCBI Accession #: NP_038474.2; NP_001138597.1
• NCBI GenBank Nucleotide #: NM_013446.3
• UniProt Accession #: Q9UHC7; Q256Y7; Q59G11; Q6GSF1; Q9H0G0; Q9UEZ7; Q9UHW2; A4D1T7; B3KXB4
• Molecular Weight: 35,224 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase makorin-1 isoform 1
• NCBI Official Synonym Full Names: makorin ring finger protein 1
• NCBI Official Symbol: MKRN1
• NCBI Official Synonym Symbols: RNF61
• NCBI Protein Information: E3 ubiquitin-protein ligase makorin-1
• UniProt Protein Name: E3 ubiquitin-protein ligase makorin-1
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: MKRN1
• UniProt Synonym Gene Names: RNF61
• UniProt Entry Name: MKRN1_HUMAN

NCBI Description

This gene encodes a protein that belongs to a novel class of zinc finger proteins. The encoded protein functions as a transcriptional co-regulator, and as an E3 ubiquitin ligase that promotes the ubiquitination and proteasomal degradation of target proteins. The protein encoded by this gene is thought to regulate RNA polymerase II-catalyzed transcription. Substrates for this protein's E3 ubiquitin ligase activity include the capsid protein of the West Nile virus and the catalytic subunit of the telomerase ribonucleoprotein. This protein controls cell cycle arrest and apoptosis by regulating p21, a cell cycle regulator, and the tumor suppressor protein p53. Pseudogenes of this gene are present on chromosomes 1, 3, 9, 12 and 20, and on the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2014]

Uniprot Description

MKRN1: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Ligase; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 7q34

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; chromatin binding; ligase activity

Biological Process: protein polyubiquitination