Rabbit anti-Human SEMA4A Polyclonal Antibody | anti-SEMA4A antibody

Anti-SEMA4A Antibody, Rabbit Polyclonal

Cat. Num. AAA8105782

• Gene Names: SEMA4A; RP35; SEMB; SEMAB; CORD10
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: SEMA4A; Polyclonal Antibody; Anti-SEMA4A Antibody; Rabbit Polyclonal; Semaphorin 4A/SEMA4A/Semaphorin B Antibody; Rabbit PAb; Antigen Affinity Purified; semaphorin 4A; Anti-CORD10 Antibody; Anti-RP35 Antibody; Anti-SEMAB Antibody; Anti-SEMB Antibody; anti-SEMA4A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Semaphorin 4A/SEMA4A/Semaphorin B
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SEMA4A antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000; IP: 0.2-1uL/mg of lysate
• Immunogen: Recombinant Human Semaphorin 4A/SEMA4A/Semaphorin B protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human Semaphorin 4A/SEMA4A/Semaphorin B (rh Semaphorin 4A/SEMA4A/Semaphorin B). Semaphorin 4A/SEMA4A/Semaphorin B specific IgG was purified by Human Semaphorin 4A/SEMA4A/Semaphorin B affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(SEMA4A was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-SEMA4A rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SEMA4A rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 84 kDaObserved band size: 105 kDa)

Western Blot (WB)

(Anti-SEMA4A rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:84 kDaObserved band size:84 kDa)

Related Product Information for anti-SEMA4A antibody

Semaphorin-4A, also known as Semaphorin-B, SEMA4A, Sema B and SEMAB, is a single-pass type I membrane protein that belongs to the semaphorin family. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Semaphorin-4A/SEMA4A contains one Ig-like C2-type (immunoglobulin-like) domain, one PSI domain and one Sema domain. Defects in SEMA4A are the cause of retinitis pigmentosa type 35 (RP35) which leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of mid-peripheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Defects in SEMA4A are also the cause of cone-rod dystrophy type 1 (CORD1) which are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. Semaphorins are secreted, transmembrane, and GPI-linked proteins, defined by cysteine-rich semaphorin protein domains, that have important roles in a variety of tissues. Humans have 2 semaphorins, Drosophila has five, and two are known from DNA viruses. Semaphorins are found in nematodes and crustaceans but not in non-animals. They are grouped into eight classes on the basis of phylogenetic tree analyses and the presence of additional protein motifs. Semaphorins have been implicated in diverse developmental processes such as axon guidance during nervous system development and regulation of cell migration.

References

Clark H.F., et al., 2003, Genome Res. 13: 2265-2270. Ota T., et al., 2004, Nat. Genet. 36: 40-45. Neufeld, G. et al., 2005, Front Biosci. 10 : 751-60. Fiore, R. et al., 2005, Mol Cell Biol. 25 (6):2310-9. Abid A., et al., 2006, J. Med. Genet. 43:378-381.

NCBI and Uniprot Product Information

• NCBI GI #: 21361914
• NCBI GeneID: 64218
• NCBI Accession #: NP_071762.2
• NCBI GenBank Nucleotide #: NM_022367.3
• UniProt Accession #: Q9H3S1; Q5TCI5; Q5TCJ6; Q8WUA9; B2RDH8; B3KR76
• Molecular Weight: 69,104 Da
• NCBI Official Full Name: semaphorin-4A isoform 1
• NCBI Official Synonym Full Names: semaphorin 4A
• NCBI Official Symbol: SEMA4A
• NCBI Official Synonym Symbols: RP35; SEMB; SEMAB; CORD10
• NCBI Protein Information: semaphorin-4A
• UniProt Protein Name: Semaphorin-4A
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA4A
• UniProt Synonym Gene Names: SEMAB; SEMB; Sema B
• UniProt Entry Name: SEM4A_HUMAN

NCBI Description

This gene encodes a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is an activator of T-cell-mediated immunity and suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010]

Uniprot Description

SEMA4A: a single-pass type I membrane protein that inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Defects in SEMA4A are the cause of retinitis pigmentosa type 35 and cone-rod dystrophy type 10.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protein binding; receptor activity

Biological Process: regulation of cell shape; axon guidance; negative regulation of angiogenesis; T-helper 1 cell differentiation; angiogenesis

Disease: Retinitis Pigmentosa 35; Cone-rod Dystrophy 10