Rabbit anti-Human, Mouse SELK Polyclonal Antibody | anti-SELK antibody

SELK, ID (SELK, Selenoprotein K) (Biotin)

Cat. Num. AAA6345912

• Gene Names: SELENOK; SELK; HSPC030; HSPC297
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SELK; Polyclonal Antibody; ID (SELK; Selenoprotein K) (Biotin); Selenoprotein K; anti-SELK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-SELK antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SELK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-61 amino acids from the Central region of human SELK.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SELK Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SELK antibody detected the SELK protein (arrow).)

Western Blot (WB)

(SELK Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the SELK antibody detected the SELK protein (arrow).)

Related Product Information for anti-SELK antibody

This gene encodes a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This selenoprotein is localized to the endoplasmic reticulum and is highly expressed in the heart, where it may function as an antioxidant.

Product Categories/Family for anti-SELK antibody

Antibodies; Selenoprotein

References

Lu, C., et al. FEBS Lett. 580(22):5189-5197(2006). Kryukov, G.V., et al. Science 300(5624):1439-1443(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 25014099
• NCBI GeneID: 58515
• NCBI Accession #: NP_067060.2
• NCBI GenBank Nucleotide #: NM_021237.4
• UniProt Accession #: Q9Y6D0; Q8IZQ3; Q9P085
• Molecular Weight: 10,645 Da
• NCBI Official Full Name: selenoprotein K
• NCBI Official Synonym Full Names: selenoprotein K
• NCBI Official Symbol: SELENOK
• NCBI Official Synonym Symbols: SELK; HSPC030; HSPC297
• NCBI Protein Information: selenoprotein K
• UniProt Protein Name: Selenoprotein K
• Protein Family: Selenoprotein
• UniProt Gene Name: SELK
• UniProt Synonym Gene Names: SelK
• UniProt Entry Name: SELK_HUMAN

NCBI Description

The protein encoded by this gene belongs to the selenoprotein K family. It is a transmembrane protein that is localized in the endoplasmic reticulum (ER), and is involved in ER-associated degradation (ERAD) of misfolded, glycosylated proteins. It also has a role in the protection of cells from ER stress-induced apoptosis. Knockout studies in mice show the importance of this gene in promoting Ca(2+) flux in immune cells and mounting effective immune response. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Pseudogenes of this locus have been identified on chromosomes 6 and 19.[provided by RefSeq, Aug 2017]

Uniprot Description

SELK: a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This selenoprotein is localized to the endoplasmic reticulum and is highly expressed in the heart, where it may function as an antioxidant. [provided by RefSeq, Jul 2008]

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane; plasma membrane

Molecular Function: identical protein binding

Biological Process: calcium ion transport; response to oxidative stress