Rabbit SEK1 Polyclonal Antibody | anti-SEK1 antibody

SEK1 antibody

Cat. Num. AAA5301946

• Gene Names: Map2k4; MEK4; MKK4; Sek1; JNKK1; Serk1; PRKMK4
• Applications: Immunohistochemistry, Western Blot
• Purity: SEK1 antibody was purified by immunoaffinity chromatography.
• Synonyms: SEK1; Polyclonal Antibody; SEK1 antibody; Polyclonal SEK1; Anti-SEK1; JNKK; SEK 1; SEK-1; Jun kinase kinase; Ab 80; anti-SEK1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Purity/Purification: SEK1 antibody was purified by immunoaffinity chromatography.
• Form/Format: Supplied in liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 397

• Applicable Applications for anti-SEK1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; WB: 1:500-1:1000
• Biological Significance: SEK1 activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflamatory cytokines. Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop. Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction.
• Cross-Reactivity: Human, Mouse, Rat
• Immunogen: SEK1 antibody was raised in Rabbit using the peptide sequence around aa 78~82 (T-H-S-I-E) derived from Human SEK1/MKK4 as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SEK1/MKK4 antibody (left) or the same antibody preincubated with with blocking peptide (right))

Western Blot (WB)

(Western Blot analysis of extracts from PC12 and C2C12 cells using SEK1/MKK4 antibody)

Related Product Information for anti-SEK1 antibody

Rabbit polyclonal SEK1 antibody

Product Categories/Family for anti-SEK1 antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 1754531
• NCBI GeneID: 26398
• Molecular Weight: 44,114 Da
• NCBI Official Full Name: SEK1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 4
• NCBI Official Symbol: Map2k4
• NCBI Official Synonym Symbols: MEK4; MKK4; Sek1; JNKK1; Serk1; PRKMK4
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 4
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 4
• UniProt Gene Name: Map2k4
• UniProt Synonym Gene Names: Jnkk1; Mek4; Mkk4; Prkmk4; Sek1; Serk1; Skk1; MAP kinase kinase 4; MAPKK 4; JNK kinase 1; JNKK 1; MEK 4; SEK1
• UniProt Entry Name: MP2K4_MOUSE

Uniprot Description

MKK4: dual specificity kinase of the STE7 family that phosphorylates and activates JNK1 and -2 as well as p38 but not ERK1 or -2. Mediates cellular responses to various cellular stresses and inflammatory cytokines. Phosphorylation by Akt inhibits MKK4 and suppresses stress-activated signal transduction.

Protein type: Protein kinase, STE; EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); Kinase, protein; STE group; STE7 family

Cellular Component: dendrite cytoplasm; axon; cytoplasm; perikaryon; intracellular; nucleus; cytosol

Molecular Function: MAP kinase kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; mitogen-activated protein kinase kinase kinase binding; kinase activity; JUN kinase kinase activity; ATP binding; protein kinase activity

Biological Process: positive regulation of apoptosis; apoptosis; positive regulation of neuron apoptosis; MAPKKK cascade; JNK cascade; positive regulation of protein amino acid phosphorylation; protein amino acid phosphorylation; phosphorylation; positive regulation of DNA replication; activation of JNK activity; positive regulation of nitric-oxide synthase biosynthetic process