Rabbit anti-Human, Mouse SECISBP2 Polyclonal Antibody | anti-SECISBP2 antibody

SECISBP2 Polyclonal Antibody

Cat. Num. AAA3011704

• Gene Names: SECISBP2; SBP2
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SECISBP2; Polyclonal Antibody; SECISBP2 Polyclonal Antibody; Selenocysteine insertion sequence-binding protein 2; SECIS-binding protein 2; SBP2; anti-SECISBP2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SECISBP2 polyclonal antibody detects endogenous levels of SECISBP2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 854

• Applicable Applications for anti-SECISBP2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human SECISBP2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cells, using SECISBP2 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver injury using SECISBP2 antibody at dilution of 1:200 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon carcinoma using SECISBP2 antibody at dilution of 1:200 (40x lens).)

Related Product Information for anti-SECISBP2 antibody

Eukaryotes require a selenocysteine (Sec) insertion sequence (SECIS) element in the 3' untranslated region of the mRNA to decode the UGA codon as Sec. SECIS-binding protein 2 (SBP-2) specifically binds selenoprotein mRNAs to form a functional complex and is essential for the insertion of Sec into selenoproteins. Purified SBP-2 interacts specifically with the SECIS element in the phospholipid hydroperoxide glutathione peroxidase mRNA. SBP-2 shows binding activity in the liver and testis as well as hepatoma cells. SBP-2 binds to a conserved RNA binding domain shared with several ribosomal proteins and eukaryotic translation termination release factor 1. A second domain located N-terminal to the RNA binding domain required for Sec insertion allows SBP-2 to stably associate with the ribosomal fraction of cells. SBP-2 preferentially stimulates incorporation directed by the selenoprotein P and phospholipid hydroperoxide glutathione peroxidase SECIS elements. SBP-2 may have a distinct function in selecting the ribosomes for Sec insertion.

NCBI and Uniprot Product Information

• NCBI GI #: 52788293
• NCBI GeneID: 79048
• UniProt Accession #: Q96T21
• Molecular Weight: ~ 110kDa
• NCBI Official Full Name: Selenocysteine insertion sequence-binding protein 2
• NCBI Official Synonym Full Names: SECIS binding protein 2
• NCBI Official Symbol: SECISBP2
• NCBI Official Synonym Symbols: SBP2
• NCBI Protein Information: selenocysteine insertion sequence-binding protein 2
• UniProt Protein Name: Selenocysteine insertion sequence-binding protein 2
• Protein Family: Selenocysteine insertion sequence-binding protein
• UniProt Gene Name: SECISBP2
• UniProt Synonym Gene Names: SBP2; SECIS-binding protein 2
• UniProt Entry Name: SEBP2_HUMAN

NCBI Description

The protein encoded by this gene is one of the essential components of the machinery involved in co-translational insertion of selenocysteine (Sec) into selenoproteins. Sec is encoded by the UGA codon, which normally signals translation termination. The recoding of UGA as Sec codon requires a Sec insertion sequence (SECIS) element; present in the 3' untranslated regions of eukaryotic selenoprotein mRNAs. This protein specifically binds to the SECIS element, which is stimulated by a Sec-specific translation elongation factor. Mutations in this gene have been associated with reduction in enzymatic activity of type II iodothyronine deiodinase (a selenoprotein) and abnormal thyroid hormone metabolism. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2017]

Uniprot Description

SECISBP2: Binds to the SECIS element in the 3'-UTR of some mRNAs encoding selenoproteins. Binding is stimulated by SELB. Defects in SECISBP2 are a cause of abnormal thyroid hormone metabolism (ATHYHM). This phenotype is associated with a reduction in type II iodothyronine deiodinase activity. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation

Chromosomal Location of Human Ortholog: 9q22.2

Cellular Component: nucleoplasm; mitochondrion; ribonucleoprotein complex

Molecular Function: ribonucleoprotein binding; mRNA 3'-UTR binding

Biological Process: selenocysteine incorporation

Disease: Thyroid Hormone Metabolism, Abnormal