Rabbit anti-Human SECISBP2 Polyclonal Antibody | anti-SECISBP2 antibody

SECISBP2, ID (SECISBP2, SBP2, Selenocysteine insertion sequence-binding protein 2) (PE)

Cat. Num. AAA6345865

• Gene Names: SECISBP2; SBP2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SECISBP2; Polyclonal Antibody; ID (SECISBP2; SBP2; Selenocysteine insertion sequence-binding protein 2) (PE); Selenocysteine insertion sequence-binding protein 2; anti-SECISBP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SECISBP2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SECISBP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 500-530 amino acids from the Central region of human SECISBP2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SECISBP2 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the SECISBP2 antibody detected the SECISBP2 protein (arrow).)

Related Product Information for anti-SECISBP2 antibody

The incorporation of selenocysteine into a protein requires the concerted action of an mRNA element called a sec insertion sequence (SECIS), a selenocysteine-specific translation elongation factor and a SECIS binding protein. With these elements in place, a UGA codon can be decoded as selenocysteine. The gene described in this record encodes a nuclear protein that functions as a SECIS binding protein. Mutations in this gene have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism.

Product Categories/Family for anti-SECISBP2 antibody

Antibodies; Binding Proteins

References

Meplan, C., et al. Carcinogenesis 31(6):1074-1079(2010). Papp, L.V., et al. Antioxid. Redox Signal. 12(7):797-808(2010). Di Cosmo, C., et al. J. Clin. Endocrinol. Metab. 94(10):4003-4009(2009). Olieric, V., et al. Biochimie 91(8):1003-1009(2009). Schomburg, L., et al. Thyroid 19(3):277-281(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 83779010
• NCBI GeneID: 79048
• NCBI Accession #: NP_076982.3
• NCBI GenBank Nucleotide #: NM_024077.4
• UniProt Accession #: Q96T21; Q5HYY1; Q7L1Z0; Q8IYC0; Q9H0A1; F8W892
• Molecular Weight: 87,985 Da
• NCBI Official Full Name: selenocysteine insertion sequence-binding protein 2 isoform 1
• NCBI Official Synonym Full Names: SECIS binding protein 2
• NCBI Official Symbol: SECISBP2
• NCBI Official Synonym Symbols: SBP2
• NCBI Protein Information: selenocysteine insertion sequence-binding protein 2
• UniProt Protein Name: Selenocysteine insertion sequence-binding protein 2
• Protein Family: Selenocysteine insertion sequence-binding protein
• UniProt Gene Name: SECISBP2
• UniProt Synonym Gene Names: SBP2; SECIS-binding protein 2
• UniProt Entry Name: SEBP2_HUMAN

NCBI Description

The incorporation of selenocysteine into a protein requires the concerted action of an mRNA element called a sec insertion sequence (SECIS), a selenocysteine-specific translation elongation factor and a SECIS binding protein. With these elements in place, a UGA codon can be decoded as selenocysteine. The gene described in this record encodes a nuclear protein that functions as a SECIS binding protein. Mutations in this gene have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

SECISBP2: Binds to the SECIS element in the 3'-UTR of some mRNAs encoding selenoproteins. Binding is stimulated by SELB. Defects in SECISBP2 are a cause of abnormal thyroid hormone metabolism (ATHYHM). This phenotype is associated with a reduction in type II iodothyronine deiodinase activity. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation

Chromosomal Location of Human Ortholog: 9q22.2

Cellular Component: nucleoplasm; mitochondrion; ribonucleoprotein complex

Molecular Function: ribonucleoprotein binding; mRNA 3'-UTR binding

Biological Process: selenocysteine incorporation

Disease: Thyroid Hormone Metabolism, Abnormal