Rabbit anti-Human SECISBP2 Polyclonal Antibody | anti-SECISBP2 antibody

SECISBP2, ID (SECISBP2, SBP2, Selenocysteine insertion sequence-binding protein 2) (APC)

Cat. Num. AAA6345856

• Gene Names: SECISBP2; SBP2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SECISBP2; Polyclonal Antibody; ID (SECISBP2; SBP2; Selenocysteine insertion sequence-binding protein 2) (APC); Selenocysteine insertion sequence-binding protein 2; anti-SECISBP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-SECISBP2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SECISBP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 500-530 amino acids from the Central region of human SECISBP2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SECISBP2 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the SECISBP2 antibody detected the SECISBP2 protein (arrow).)

Related Product Information for anti-SECISBP2 antibody

The incorporation of selenocysteine into a protein requires the concerted action of an mRNA element called a sec insertion sequence (SECIS), a selenocysteine-specific translation elongation factor and a SECIS binding protein. With these elements in place, a UGA codon can be decoded as selenocysteine. The gene described in this record encodes a nuclear protein that functions as a SECIS binding protein. Mutations in this gene have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism.

Product Categories/Family for anti-SECISBP2 antibody

Antibodies; Binding Proteins

References

Meplan, C., et al. Carcinogenesis 31(6):1074-1079(2010). Papp, L.V., et al. Antioxid. Redox Signal. 12(7):797-808(2010). Di Cosmo, C., et al. J. Clin. Endocrinol. Metab. 94(10):4003-4009(2009). Olieric, V., et al. Biochimie 91(8):1003-1009(2009). Schomburg, L., et al. Thyroid 19(3):277-281(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 83779010
• NCBI GeneID: 79048
• NCBI Accession #: NP_076982.3
• NCBI GenBank Nucleotide #: NM_024077.4
• UniProt Accession #: Q96T21; Q5HYY1; Q7L1Z0; Q8IYC0; Q9H0A1; F8W892
• Molecular Weight: 87,985 Da
• NCBI Official Full Name: selenocysteine insertion sequence-binding protein 2 isoform 1
• NCBI Official Synonym Full Names: SECIS binding protein 2
• NCBI Official Symbol: SECISBP2
• NCBI Official Synonym Symbols: SBP2
• NCBI Protein Information: selenocysteine insertion sequence-binding protein 2
• UniProt Protein Name: Selenocysteine insertion sequence-binding protein 2
• Protein Family: Selenocysteine insertion sequence-binding protein
• UniProt Gene Name: SECISBP2
• UniProt Synonym Gene Names: SBP2; SECIS-binding protein 2
• UniProt Entry Name: SEBP2_HUMAN

NCBI Description

The incorporation of selenocysteine into a protein requires the concerted action of an mRNA element called a sec insertion sequence (SECIS), a selenocysteine-specific translation elongation factor and a SECIS binding protein. With these elements in place, a UGA codon can be decoded as selenocysteine. The gene described in this record encodes a nuclear protein that functions as a SECIS binding protein. Mutations in this gene have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

SECISBP2: Binds to the SECIS element in the 3'-UTR of some mRNAs encoding selenoproteins. Binding is stimulated by SELB. Defects in SECISBP2 are a cause of abnormal thyroid hormone metabolism (ATHYHM). This phenotype is associated with a reduction in type II iodothyronine deiodinase activity. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation

Chromosomal Location of Human Ortholog: 9q22.2

Cellular Component: nucleoplasm; mitochondrion; ribonucleoprotein complex

Molecular Function: ribonucleoprotein binding; mRNA 3'-UTR binding

Biological Process: selenocysteine incorporation

Disease: Thyroid Hormone Metabolism, Abnormal