Rabbit anti-Human, Mouse SEC61B Polyclonal Antibody | anti-SEC61B antibody

SEC61B, ID (SEC61B, Protein transport protein Sec61 subunit beta) (PE)

Cat. Num. AAA6345832

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SEC61B; Polyclonal Antibody; ID (SEC61B; Protein transport protein Sec61 subunit beta) (PE); Protein transport protein Sec61 subunit beta; anti-SEC61B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SEC61B antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SEC61B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the Central region of human SEC61B.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SEC61B Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the SEC61B antibody detected the SEC61B protein (arrow).)

Immunohistochemistry (IHC)

(SEC61B Antibody (Center) (AP14910c)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SEC61B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-SEC61B antibody

The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins-alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript.

Product Categories/Family for anti-SEC61B antibody

Antibodies; Transport Proteins

References

Liao, H.J., et al. Cancer Res. 69(15):6179-6183(2009). Wang, B., et al. Cell 133(6):1080-1092(2008). Liao, H.J., et al. Mol. Biol. Cell 18(3):1064-1072(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 5803165
• NCBI GeneID: 10952
• NCBI Accession #: NP_006799.1
• NCBI GenBank Nucleotide #: NM_006808.2
• UniProt Accession #: P60468; P38390; P38391; Q6IBC1
• Molecular Weight: 9,974 Da
• NCBI Official Full Name: protein transport protein Sec61 subunit beta
• NCBI Official Synonym Full Names: Sec61 beta subunit
• NCBI Official Symbol: SEC61B
• NCBI Protein Information: protein transport protein Sec61 subunit beta; Sec61 complex, beta subunit; protein translocation complex beta; protein transport protein SEC61 beta subunit
• UniProt Protein Name: Protein transport protein Sec61 subunit beta
• Protein Family: Protein transport protein
• UniProt Gene Name: SEC61B
• UniProt Entry Name: SC61B_HUMAN

NCBI Description

The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript. [provided by RefSeq, Jul 2008]

Uniprot Description

SEC61B: Necessary for protein translocation in the endoplasmic reticulum. Belongs to the SEC61-beta family.

Protein type: Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q22.33

Cellular Component: membrane; endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; epidermal growth factor binding

Biological Process: retrograde protein transport, ER to cytosol; SRP-dependent cotranslational protein targeting to membrane; antigen processing and presentation of peptide antigen via MHC class I; ER-associated protein catabolic process; cellular protein metabolic process; translation; protein import into nucleus, translocation; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression