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Western Blot (WB) (SEC22B Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SEC22B antibody detected the SEC22B protein (arrow).)

Rabbit anti-Human SEC22B Polyclonal Antibody | anti-SEC22B antibody

SEC22B, ID (SEC22B, SEC22L1, Vesicle-trafficking protein SEC22b, ER-Golgi SNARE of 24kD, SEC22 vesicle-trafficking protein homolog B, SEC22 vesicle-trafficking protein-like 1) (Azide free) (HRP)

Gene Names
SEC22B; ERS-24; SEC22L1
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SEC22B; Polyclonal Antibody; ID (SEC22B; SEC22L1; Vesicle-trafficking protein SEC22b; ER-Golgi SNARE of 24kD; SEC22 vesicle-trafficking protein homolog B; SEC22 vesicle-trafficking protein-like 1) (Azide free) (HRP); SEC22 vesicle-trafficking protein-like 1; anti-SEC22B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Sequence Length
215
Applicable Applications for anti-SEC22B antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
SEC22B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-104 amino acids from the Central region of human SEC22B.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SEC22B Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SEC22B antibody detected the SEC22B protein (arrow).)

Western Blot (WB) (SEC22B Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SEC22B antibody detected the SEC22B protein (arrow).)

Immunohistochemistry (IHC)

(SEC22B Antibody (Center) (AP14062c)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SEC22B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (SEC22B Antibody (Center) (AP14062c)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SEC22B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-SEC22B antibody
The protein encoded by this gene is a member of the SEC22 family of vesicle trafficking proteins. It seems to complex with SNARE and it is thought to play a role in the ER-Golgi protein trafficking. This protein has strong similarity to Mus musculus and Cricetulus griseus proteins.
Product Categories/Family for anti-SEC22B antibody
References
Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Chi, A., et al. J. Proteome Res. 5(11):3135-3144(2006). Okumura, A.J., et al. J. Biol. Chem. 281(7):4495-4506(2006). Breuza, L., et al. J. Biol. Chem. 279(45):47242-47253(2004). Nakajima, K., et al. EMBO J. 23(16):3216-3226(2004).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
vesicle-trafficking protein SEC22b
NCBI Official Synonym Full Names
SEC22 homolog B, vesicle trafficking protein (gene/pseudogene)
NCBI Official Symbol
SEC22B
NCBI Official Synonym Symbols
ERS-24; SEC22L1
NCBI Protein Information
vesicle-trafficking protein SEC22b
UniProt Protein Name
Vesicle-trafficking protein SEC22b
UniProt Gene Name
SEC22B
UniProt Synonym Gene Names
SEC22L1; ERS-24; ERS24
UniProt Entry Name
SC22B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the SEC22 family of vesicle trafficking proteins. It seems to complex with SNARE and it is thought to play a role in the ER-Golgi protein trafficking. This protein has strong similarity to Mus musculus and Cricetulus griseus proteins.[provided by RefSeq, Sep 2009]

Uniprot Description

SEC22B: SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER. Belongs to the synaptobrevin family.

Protein type: Vesicle; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; integral to membrane; ER-Golgi intermediate compartment; melanosome

Molecular Function: protein binding; syntaxin binding

Biological Process: ER to Golgi vesicle-mediated transport; protein transport; positive regulation of protein catabolic process; regulation of organelle organization and biogenesis

Research Articles on SEC22B

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Product Notes

The SEC22B sec22b (Catalog #AAA6345760) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SEC22B, ID (SEC22B, SEC22L1, Vesicle-trafficking protein SEC22b, ER-Golgi SNARE of 24kD, SEC22 vesicle-trafficking protein homolog B, SEC22 vesicle-trafficking protein-like 1) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SEC22B can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SEC22B sec22b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SEC22B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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