Western Blot (WB)
(Western blot analysis of Scribble in lysates of HCT 116, using Scribble Antibody(AF4771).)
Rabbit Scribble Polyclonal Antibody | anti-SCRIB antibody
Scribble Antibody
Gene Names
SCRIB; CRIB1; SCRB1; SCRIB1; Vartul
Reactivity
Human, MousePredicted Reactivity: Pig (83%), Bovine (83%), Horse (92%)
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
Scribble; Polyclonal Antibody; Scribble Antibody; CRIB 1; CRIB1; hScrib; LAP 4; LAP4; PDZ domain protein scribble; Protein LAP4; Protein scribble homolog; SCRB 1; SCRB1; SCRIB 1; Scrib; SCRIB_HUMAN; SCRIB1; Scribble homolog 1; scribbled homolog (Drosophila); Vartul; anti-SCRIB antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted Reactivity: Pig (83%), Bovine (83%), Horse (92%)
Predicted Reactivity: Pig (83%), Bovine (83%), Horse (92%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Scribble Antibody detects endogenous levels of total Scribble.
Tissue Specificity: Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).
Tissue Specificity: Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-SCRIB antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human Scribble, corresponding to a region within the internal amino acids.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Ubiquitinated; targeted for UBE3A-dependent multiubiquitination in the presence of high-risk HPV E6 proteins and degraded.
Subunit Structure
Interacts (via PDZ domains) with VANGL2. Interacts with CTNNB1 and MAPK12 (By similarity). Interacts with UBE3A and HPV E6. Interacts with PAK1 and PAK2. Interacts with ARHGEF7 and GIT1; interacts directly with ARHGEF7. Interacts (via PDZ domains) with LPP and TRIP6; the interaction is direct. Interacts (via PDZ domains) with TJP2. Interacts (via PDZ domains) with APC; may mediate APC targeting to adherens junctions of epithelial cells. Interacts (via PDZ domains) with TSHR; regulates TSHR trafficking and function. Interacts (via PDZ domains 1 and 3) with MCC. Interacts (via fourth PDZ domain) with tick-borne encephalitis virus NS5 protein; this interaction inhibits SCRIB and downstream STAT1 phosphorylation, thereby preventing activation of JAK-STAT signaling pathway. Interacts with DLG5. Interacts with STK4/MST1 and LATS1 in the presence of DLG5. (Microbial infection) Interacts with tick-borne encephalitis virus RNA-directed RNA polymerase NS5; this interaction targets viral NS5 to the cell membrane periphery and nucleus, thereby allowing efficient host nuclear STAT1 inhibition.
Similarity
Belongs to the LAP (LRR and PDZ) protein family.
Subcellular Location
Cell membrane>Peripheral membrane protein. Cell junction>Adherens junction. Cell projection>Lamellipodium. Cytoplasm. Cell junction>Synapse>Postsynapse. Cell junction>Synapse>Presynapse.
Note: Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity.
Note: Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of Scribble in lysates of HCT 116, using Scribble Antibody(AF4771).)
Western Blot (WB)
(Western blot analysis of Scribble in lysates of HCT 116, using Scribble Antibody(AF4771).)
Related Product Information for anti-SCRIB antibody
Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
177,694 Da
NCBI Official Full Name
protein scribble homolog isoform b
NCBI Official Synonym Full Names
scribbled planar cell polarity protein
NCBI Official Symbol
SCRIB
NCBI Official Synonym Symbols
CRIB1; SCRB1; SCRIB1; Vartul
NCBI Protein Information
protein scribble homolog; scribbled homolog
UniProt Protein Name
Protein scribble homolog
Protein Family
UniProt Gene Name
SCRIB
UniProt Synonym Gene Names
CRIB1; KIAA0147; LAP4; SCRB1; VARTUL; Scribble; hScrib
UniProt Entry Name
SCRIB_HUMAN
Similar Products
Product Notes
The SCRIB scrib (Catalog #AAA9613478) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Scribble Antibody reacts with Human, Mouse Predicted Reactivity: Pig (83%), Bovine (83%), Horse (92%) and may cross-react with other species as described in the data sheet. AAA Biotech's Scribble can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the SCRIB scrib for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Scribble, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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