Rabbit anti-Human, Mouse SCARF2 Polyclonal Antibody | anti-SCARF2 antibody

SCARF2, CT (SCARF2, SREC2, SREPCR, Scavenger receptor class F member 2, SRECRP-1, Scavenger receptor expressed by endothelial cells 2 protein) (FITC)

Cat. Num. AAA6345242

• Gene Names: SCARF2; NSR1; SREC2; VDEGS; SREC-II; SRECRP-1
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SCARF2; Polyclonal Antibody; CT (SCARF2; SREC2; SREPCR; Scavenger receptor class F member 2; SRECRP-1; Scavenger receptor expressed by endothelial cells 2 protein) (FITC); Scavenger receptor expressed by endothelial cells 2 protein; anti-SCARF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SCARF2 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SCARF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 829-858 amino acids from the C-terminal region of human SCARF2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SCARF2 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the SCARF2 antibody detected the SCARF2 protein (arrow).)

Flow Cytometry (FC/FACS)

(SCARF2 Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SCARF2 antibody

The protein encoded by this gene is similar to SCARF1/SREC-I, a scavenger receptor protein that mediates the binding and degradation of acetylated low density lipoprotein (Ac-LDL). This protein has only little activity of internalizing modified low density lipoproteins (LDL), but it can interact with SCARF1 through its extracellular domain. The association of this protein with SCARF1 is suppressed by the presence of scavenger ligands. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Product Categories/Family for anti-SCARF2 antibody

Antibodies; Receptors; Scavenger

References

Anastasio, N., et al. Am. J. Hum. Genet. 87(4):553-559(2010). Wu, C., et al. Proteomics 7(11):1775-1785(2007). Ishii, J., et al. J. Biol. Chem. 277(42):39696-39702(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 33598937
• NCBI GeneID: 91179
• NCBI Accession #: NP_878315.1; NP_699165.2
• NCBI GenBank Nucleotide #: NM_182895.2
• UniProt Accession #: Q96GP6; Q58A83; Q8IXF3; Q9BW74; E5RFB8
• Molecular Weight: 92,479 Da
• NCBI Official Full Name: scavenger receptor class F member 2 isoform 2
• NCBI Official Synonym Full Names: scavenger receptor class F, member 2
• NCBI Official Symbol: SCARF2
• NCBI Official Synonym Symbols: NSR1; SREC2; VDEGS; SREC-II; SRECRP-1
• NCBI Protein Information: scavenger receptor class F member 2; scavenger receptor expressed by endothelial cells 2 protein
• UniProt Protein Name: Scavenger receptor class F member 2
• Protein Family: Scavenger receptor
• UniProt Gene Name: SCARF2
• UniProt Synonym Gene Names: SREC2; SREPCR; SREC-II
• UniProt Entry Name: SREC2_HUMAN

NCBI Description

The protein encoded by this gene is similar to SCARF1/SREC-I, a scavenger receptor protein that mediates the binding and degradation of acetylated low density lipoprotein (Ac-LDL). This protein has only little activity of internalizing modified low density lipoproteins (LDL), but it can interact with SCARF1 through its extracellular domain. The association of this protein with SCARF1 is suppressed by the presence of scavenger ligands. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

SREC-II: Probable adhesion protein, which mediates homophilic and heterophilic interactions. In contrast to SCARF1, it poorly mediates the binding and degradation of acetylated low density lipoprotein (Ac-LDL). Defects in SCARF2 are the cause of Van den Ende-Gupta syndrome (VDEGS). A syndrome characterized by craniofacial and skeletal abnormalities that include blepharophimosis, a flat and wide nasal bridge, narrow and beaked nose, hypoplastic maxilla with or without cleft palate and everted lower lip, prominent ears, down-slanting eyes, arachnodactyly, and camptodactyly. Patients present congenital joint contractures that improve without intervention, and normal growth and development. Intelligence is normal. Rarely, enlarged cerebella can be present. Some patients experience respiratory problems due to laryngeal abnormalities. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: focal adhesion; integral to membrane

Molecular Function: protein binding; scavenger receptor activity

Biological Process: heterophilic cell adhesion; receptor-mediated endocytosis

Disease: Van Den Ende-gupta Syndrome