Rabbit SATB1 Polyclonal Antibody | anti-SATB1 antibody

SATB1 Antibody

Cat. Num. AAA151179

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: SATB1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: SATB1; Polyclonal Antibody; SATB1 Antibody; DNA-binding protein SATB1; Special AT-rich sequence-binding protein 1; SATB homeobox 1; anti-SATB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: SATB1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 763

• Applicable Applications for anti-SATB1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: SATB1 antibody can be used for detection of SATB1 by Western blot at 2 - 4 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: SATB1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human SATB1.
• Buffer: SATB1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: SATB1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of SATB1 in A20 cell lysate with SATB1 antibody at (A) 2 and (B) 4 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of SATB1 in human brain with SATB1 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of SATB1 in Human Brain cells with SATB1 antibody at 20 μg/mL.)

Related Product Information for anti-SATB1 antibody

SATB1 Antibody: Human special AT-rich sequence-binding protein-1 (SATB1) is a nuclear matrix/scaffold-associated region DNA-binding protein, predominantly expressed in the thymus and pre-B cells. Like its homolog SATB2, SATB1 selectively binds double-stranded, special AT-rich DNA sequences in which one strand exclusively consists of well-mixed A, T, and C nucleotides. SATB1 contains a dimerization domain that shares similarity with the PDZ motif, identified as an indispensable element for high-affinity binding of SATB1 to DNA. SATB1 constitutes a functional nuclear architecture that has a 'cage-like' protein distribution surrounding heterochromatin and regulates gene expression through chromatin remodeling/HDAC (histone deacetylase complex) and transcription factors recruitment. SATB1 functions as a 'genome organizer' essential for proper T-cell development. Recent studies show that SATB1 is necessary for breast cancer cells to become metastatic, and when ectopically expressed in non-metastatic cells, can induce invasive activity in vivo. At least two isoforms of SATB1 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 417747
• NCBI GeneID: 6304
• UniProt Accession #: Q01826; Q59EQ0; B3KXF1; C9JTR6
• Molecular Weight: 89,127 Da
• NCBI Official Full Name: DNA-binding protein SATB1
• NCBI Official Synonym Full Names: SATB homeobox 1
• NCBI Official Symbol: SATB1
• NCBI Protein Information: DNA-binding protein SATB1; special AT-rich sequence-binding protein 1; special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA)
• UniProt Protein Name: DNA-binding protein SATB1
• Protein Family: DNA-binding protein
• UniProt Gene Name: SATB1
• UniProt Entry Name: SATB1_HUMAN

NCBI Description

This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]

Uniprot Description

SATB1: Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma. Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1- binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly up-regulating metastasis-associated genes while down-regulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis. Interacts (via DNA-binding domains) with CUX1; leading to inhibit the attachment to DNA. Homodimer. Part of the nuclear protein complex gamma-globin promoter and enhancer binding factor (gamma-PE) composed at least by SATB1 and HOXB2. Interaction with CtBP1 when not acetylated stabalizes attachment to DNA and promotes transcription repression. Interacts with PCAF. Interacts with sumoylated PML, HDAC1 and HIV-1 Tat via the PDZ- like dimerization domain. Interacts also with DYNLT3 and POLR2J2. Binds to EP300. Expressed predominantly in thymus. Belongs to the CUT homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 3p23

Cellular Component: nucleoplasm; PML body; nuclear matrix; nuclear heterochromatin; nucleus

Molecular Function: protein binding; double-stranded DNA binding; chromatin binding

Biological Process: establishment and/or maintenance of chromatin architecture; epidermis development; CD8-positive, alpha-beta T cell differentiation; CD4-positive, alpha beta T cell differentiation; viral reproduction; transcription, DNA-dependent; apoptosis; activated T cell proliferation; negative regulation of transcription from RNA polymerase II promoter; histone methylation; chromatin remodeling; reflex; cell structure disassembly during apoptosis