Rabbit SARS virus PUPM Polyclonal Antibody | anti-M antibody
SARS virus PUPM Antibody (C-term)
Reactivity
SARS
Applications
ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SARS virus PUPM; Polyclonal Antibody; SARS virus PUPM Antibody (C-term); Membrane protein; M protein; E1 glycoprotein; Matrix glycoprotein; Membrane glycoprotein; M; anti-M antibody
Host
Rabbit
Reactivity
SARS
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SARS virus PUPM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192~221 amino acids from the C-terminus region of SARS M protein.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
192-221
Sequence Length
221
Applicable Applications for anti-M antibody
ELISA (EIA)
Antigen Type
Synthetic Peptide
Antigen Source
CVHSA
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Related Product Information for anti-M antibody
An outbreak of atypical pneumonia, referred to as severe acute respiratory syndrome (SARS) and first identified in Guangdong Province, China, has spread to several countries. The severity of this disease is such that the mortality rate appears to be ~3 to 6%. A number of laboratories worldwidehave undertaken the identification of the causative agent. The National Microbiology Laboratory in Canada obtained the Tor2 isolate from a patient in Toronto, and succeeded in growing a coronavirus-like agent in African
Green Monkey Kidney (Vero E6) cells. This coronavirus has been named publicly by the World Health Organization and member laboratories as ?SARS virus?
The SARS membrane proteins, including the major proteins S (Spike) and M (Membrane), are inserted into the endoplasmic reticulum Golgi intermediate compartment (ERGIC) while full length replicated RNA (+ strands) assemble with the N (nucleocapsid) protein. The virus then migrates through the Golgi complex and eventually exits the cell, likely by exocytosis. The site of viral attachment to the host cell resides within the S protein.
Oligomeric spike (S) glycoproteins extend from SARS membranes. These integral membrane proteins assemble within the endoplasmic reticulum of infected cells and are subsequently endoproteolyzed in the Golgi, generating noncovalently associated S1 and S2 fragments. Once on the surface of infected cells and virions, peripheral S1 fragments bind carcinoembryonic antigen-related cell adhesion molecule (CEACAM) receptors, and this triggers membrane fusion reactions mediated by integral membrane S2 fragments.
Green Monkey Kidney (Vero E6) cells. This coronavirus has been named publicly by the World Health Organization and member laboratories as ?SARS virus?
The SARS membrane proteins, including the major proteins S (Spike) and M (Membrane), are inserted into the endoplasmic reticulum Golgi intermediate compartment (ERGIC) while full length replicated RNA (+ strands) assemble with the N (nucleocapsid) protein. The virus then migrates through the Golgi complex and eventually exits the cell, likely by exocytosis. The site of viral attachment to the host cell resides within the S protein.
Oligomeric spike (S) glycoproteins extend from SARS membranes. These integral membrane proteins assemble within the endoplasmic reticulum of infected cells and are subsequently endoproteolyzed in the Golgi, generating noncovalently associated S1 and S2 fragments. Once on the surface of infected cells and virions, peripheral S1 fragments bind carcinoembryonic antigen-related cell adhesion molecule (CEACAM) receptors, and this triggers membrane fusion reactions mediated by integral membrane S2 fragments.
Product Categories/Family for anti-M antibody
References
He, R., et al., Biochem. Biophys. Res. Commun. 316(2):476-483 (2004).
Zhang, X.L., et al., Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 35(12):1140-1144 (2003).
Snijder, E.J., et al., J. Mol. Biol. 331(5):991-1004 (2003).
Marra, M.A., et al., Science 300(5624):1399-1404 (2003).
Zhang, X.L., et al., Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 35(12):1140-1144 (2003).
Snijder, E.J., et al., J. Mol. Biol. 331(5):991-1004 (2003).
Marra, M.A., et al., Science 300(5624):1399-1404 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25061
NCBI Official Full Name
matrix protein
NCBI Official Symbol
M
NCBI Protein Information
matrix protein
UniProt Protein Name
Membrane protein
UniProt Gene Name
M
UniProt Synonym Gene Names
M protein
UniProt Entry Name
VME1_CVHSA
Uniprot Description
Component of the viral envelope that plays a central role in virus morphogenesis and assembly via its interactions with other viral proteins.
Research Articles on M
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Product Notes
The M m (Catalog #AAA9208907) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 192-221. The SARS virus PUPM Antibody (C-term) reacts with SARS and may cross-react with other species as described in the data sheet. AAA Biotech's SARS virus PUPM can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the M m for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SARS virus PUPM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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