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Western Blot (WB) (Western blot analysis of TPI1 Antibody (N-term) in Y79(lane 1),CEM(lane 2) cell line and mouse brain tissue(lane 3) lysates (35ug/lane). TPI1 (arrow) was detected using the purified Pab.(2ug/ml))

Rabbit anti-Human, Mouse TPI1 Polyclonal Antibody | anti-TPI1 antibody

TPI1, NT (Triosephosphate Isomerase, TIM, Triose-phosphate Isomerase, TPI) (AP)

Gene Names
TPI1; TIM; TPI; TPID; HEL-S-49
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
TPI1; Polyclonal Antibody; NT (Triosephosphate Isomerase; TIM; Triose-phosphate Isomerase; TPI) (AP); anti-TPI1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human TPI1. Species Crossreactivity: mouse.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Applicable Applications for anti-TPI1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:50-1:100
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human TPI1 (KLH).
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TPI1 Antibody (N-term) in Y79(lane 1),CEM(lane 2) cell line and mouse brain tissue(lane 3) lysates (35ug/lane). TPI1 (arrow) was detected using the purified Pab.(2ug/ml))

Western Blot (WB) (Western blot analysis of TPI1 Antibody (N-term) in Y79(lane 1),CEM(lane 2) cell line and mouse brain tissue(lane 3) lysates (35ug/lane). TPI1 (arrow) was detected using the purified Pab.(2ug/ml))

Western Blot (WB)

(Western blot analysis of TPI1 (arrow) using rabbit polyclonal TPI1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TPI1 gene.)

Western Blot (WB) (Western blot analysis of TPI1 (arrow) using rabbit polyclonal TPI1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TPI1 gene.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TPI1 Antibody (N-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (TPI1 Antibody (N-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-TPI1 antibody
References
1. Martins-de-Souza, D., et.al., BMC Psychiatry 9, 17 (2009).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28.8kDa (269aa) confirmed by MALDI-TOF
NCBI Official Full Name
triosephosphate isomerase isoform 1
NCBI Official Synonym Full Names
triosephosphate isomerase 1
NCBI Official Symbol
TPI1
NCBI Official Synonym Symbols
TIM; TPI; TPID; HEL-S-49
NCBI Protein Information
triosephosphate isomerase
UniProt Protein Name
Triosephosphate isomerase
UniProt Gene Name
TPI1
UniProt Synonym Gene Names
TPI; TIM
UniProt Entry Name
TPIS_HUMAN

NCBI Description

This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

Research Articles on TPI1

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Product Notes

The TPI1 tpi1 (Catalog #AAA6503598) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TPI1, NT (Triosephosphate Isomerase, TIM, Triose-phosphate Isomerase, TPI) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TPI1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:50-1:100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TPI1 tpi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TPI1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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