Mouse Leucine-Rich Repeat Kinase 2 (LRRK2) Monoclonal Antibody | anti-LRRK2 antibody

Leucine-Rich Repeat Kinase 2 (LRRK2) Monoclonal Antibody

Cat. Num. AAA191366

• Gene Names: LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
• Applications: Immunoblot, Western Blot, Immunofluorescence
• Synonyms: Leucine-Rich Repeat Kinase 2 (LRRK2); Monoclonal Antibody; Leucine-Rich Repeat Kinase 2 (LRRK2) Monoclonal Antibody; anti-LRRK2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Specificity: This antibody recognizes human and rat LRRK2.
• Form/Format: 100ug (1mg/ml) Protein G-purified antibody in PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.
• Sequence Length: 2527

• Applicable Applications for anti-LRRK2 antibody: Immunoblot, Immunofluorescence (IF)
• Application Notes: Immunoblot: 1-5ug/ml. A band of >200kDa is detected; IF: 10ug/ml; Dilute in PBS or medium that is identical to that used in the assay system.
• Immunogen: Fusion protein corresponding to aa 841-960 of human LRRK2. This sequence is 81% identical in mouse, 80% identical in rat, and <30% identical in LRRK1.
• Preparation and Storage: This product is stable for at least one (1) year at-20 degree C.

Testing Data

(Detection of LRRK2 in rat brain lysate at 5ug/ml.)

Testing Data

(Detection of LRRK2 in neuroblastoma cell line SK-N-BE at 10ug/ml: DAPI (blue) nuclear stain, Texas Red F actin stain, ATTO 488 (green) LRRK2 stain.)

Related Product Information for anti-LRRK2 antibody

Leucine-rich repeat kinase 2 (LRRK2), also known as dardarin, is an enzyme that in humans is encoded by the PARK8 gene. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson's disease type 8 and Crohn's disease which suggests that these two diseases share a common pathway. Expression of mutant LRRK2 induced apoptotic cell death in neuroblastoma cells and in mouse cortical neurons.

NCBI and Uniprot Product Information

• NCBI GI #: 171846278
• NCBI GeneID: 120892
• NCBI Accession #: NP_940980
• NCBI GenBank Nucleotide #: NM_198578.3
• UniProt Accession #: Q5S007; Q6ZS50; Q8NCX9; A6NJU2
• Molecular Weight: 286,103 Da
• NCBI Official Full Name: leucine-rich repeat serine/threonine-protein kinase 2
• NCBI Official Synonym Full Names: leucine rich repeat kinase 2
• NCBI Official Symbol: LRRK2
• NCBI Official Synonym Symbols: PARK8; RIPK7; ROCO2; AURA17; DARDARIN
• NCBI Protein Information: leucine-rich repeat serine/threonine-protein kinase 2
• UniProt Protein Name: Leucine-rich repeat serine/threonine-protein kinase 2
• Protein Family: Leucine-rich repeat serine/threonine-protein kinase
• UniProt Gene Name: LRRK2
• UniProt Synonym Gene Names: PARK8

NCBI Description

This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq, Jul 2008]

Uniprot Description

Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). Plays a role in synaptic vesicle trafficking. Phosphorylates PRDX3. Has GTPase activity. May play a role in the phosphorylation of proteins central to Parkinson disease.