Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK (Phospho-Thr183) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Rabbit SAPK/JNK Polyclonal Antibody | anti-SAPK/JNK antibody
SAPK/JNK antibody
Applications
Immunohistochemistry, Western Blot
Purity
SAPK/JNK antibody was purified by immunoaffinity chromatography.
Synonyms
SAPK/JNK; Polyclonal Antibody; SAPK/JNK antibody; Polyclonal SAPK/JNK; Anti-SAPK/JNK; Phospho Thr183; anti-SAPK/JNK antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
SAPK/JNK antibody was purified by immunoaffinity chromatography.
Form/Format
Supplied in liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-SAPK/JNK antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC: 1:50-1:100
WB: 1:500-1:1000
WB: 1:500-1:1000
Biological Significance
Stress-activated protein kinases (SAPK)/Jun amino-terminal kinases (JNK) are members of the MAPK family and are activated by a variety of environmental stresses, inflammatory cytokines, growth factors and GPCR agonists. Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42). As with the other MAPKs, the membrane proximal kinase is a MAPKKK, typically MEKK1?4, or a member of the mixed lineage kinases (mlK) that phosphorylates and activates MKK4 (SEK) or MKK7, the SAPK/JNK kinases.
Cross-Reactivity
Human, Mouse, Rat
Immunogen
SAPK/JNK antibody was raised in Rabbit using the peptide sequence around phosphorylation site of threonine 183 (M-M-T(p)-P-Y) derived from Human SAPK/JNK as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK (Phospho-Thr183) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK (Phospho-Thr183) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Western Blot (WB)
(Western Blot analysis of extracts from 293 cells using SAPK/JNK (Phospho-Thr183) antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane 1))
Western Blot (WB)
(Western Blot analysis of extracts from 293 cells using SAPK/JNK (Phospho-Thr183) antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane 1))
Related Product Information for anti-SAPK/JNK antibody
Rabbit polyclonal SAPK/JNK antibody
Product Categories/Family for anti-SAPK/JNK antibody
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Product Notes
The SAPK/JNK (Catalog #AAA837229) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SAPK/JNK can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). IHC: 1:50-1:100 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the SAPK/JNK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAPK/JNK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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