Rabbit SAPAP3 Polyclonal Antibody | anti-DLGAP3 antibody

SAPAP3 Antibody

Cat. Num. AAA150571

• Gene Names: DLGAP3; DAP3; SAPAP3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: SAPAP3 Antibody is affinity chromatography purified via peptide column.
• Synonyms: SAPAP3; Polyclonal Antibody; SAPAP3 Antibody; DAP3; Disks large-associated protein 3; DAP-3; discs; large (Drosophila) homolog-associated protein 3; anti-DLGAP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: SAPAP3 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 979

• Applicable Applications for anti-DLGAP3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: SAPAP3 antibody can be used for detection of SAPAP3 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: SAPAP3 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human SAPAP3.
• Buffer: SAPAP3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: SAPAP3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of SAPAP3 in rat brain tissue lysate with SAPAP3 antibody at (A) 1 (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of human brain tissue using SAPAP3 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of SAPAP3 in human brain tissue with SAPAP3 antibody at 20 μg/mL.)

Related Product Information for anti-DLGAP3 antibody

SAPAP3 Antibody: SAP90/PSD-95-associated protein 3 (SAPAP3, also known as DLGAP3) is a member of a protein family whose members specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. Like the other SAPAP proteins, SAPAP3 is thought to be an adaptor protein that also interacts with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). Both SAPAP3 protein and mRNA are targeted to dendrites, whereas SAPAP1, -2, and -4 mRNAs are detected mainly in cell bodies. Recent experiments have suggested that SAPAP3 may be involved in obsessive-compulsive disorder (OCD), as mice lacking SAPAP3 exhibited OCD-like symptoms which could be relieved by lentiviral-mediated selective expression of SAPAP3 in the striatum of SAPAP3-mutant mice. At least two isoforms are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 71153509
• NCBI GeneID: 58512
• UniProt Accession #: O95886; Q5TDD5; Q9H3X7
• Molecular Weight: 106,040 Da
• NCBI Official Full Name: Disks large-associated protein 3
• NCBI Official Synonym Full Names: discs, large (Drosophila) homolog-associated protein 3
• NCBI Official Symbol: DLGAP3
• NCBI Official Synonym Symbols: DAP3; SAPAP3
• NCBI Protein Information: disks large-associated protein 3; DAP-3; PSD-95/SAP90-binding protein 3; SAP90/PSD 95 associated protein 3; SAP90/PSD-95-associated protein 3
• UniProt Protein Name: Disks large-associated protein 3
• Protein Family: Disks large-associated protein
• UniProt Gene Name: DLGAP3
• UniProt Synonym Gene Names: DAP3; DAP-3; SAPAP3
• UniProt Entry Name: DLGP3_HUMAN

Uniprot Description

SAPAP3: May play a role in the molecular organization of synapses and neuronal cell signaling. Could be an adapter protein linking ion channel to the subsynaptic cytoskeleton. May induce enrichment of PSD-95/SAP90 at the plasma membrane. Belongs to the SAPAP family.

Protein type: Membrane protein, peripheral; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p35.3-p34.1

Cellular Component: postsynaptic membrane; postsynaptic density; cell junction

Molecular Function: protein binding; beta-amyloid binding

Biological Process: cell-cell signaling