Chromatin Immunoprecipitation (ChIP)
(SAP30 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from the K-562 human myelogenous leukemia cell line (4.5 million cells) and 5 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 30 million sequence tags were mapped to identify SAP30 binding sites. The image on the left shows SAP30 binding at transcriptional start sites across a 2.4 million bp region on chromosome 1. The image on the right is zoomed in to show SAP30 binding at two other start sites.)
Rabbit anti-Human SAP30 Polyclonal Antibody | anti-SAP30 antibody
SAP30 antibody (pAb)
ChIP: 5ul per ChIP
ChIP-Seq & ChIP-chip: 5ul each
WB: 1:500 dilution
Chromatin Immunoprecipitation (ChIP)
(SAP30 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from the K-562 human myelogenous leukemia cell line (4.5 million cells) and 5 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 30 million sequence tags were mapped to identify SAP30 binding sites. The image on the left shows SAP30 binding at transcriptional start sites across a 2.4 million bp region on chromosome 1. The image on the right is zoomed in to show SAP30 binding at two other start sites.)
Chromatin Immunoprecipitation (ChIP)
(SAP30 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from the K-562 human myelogenous leukemia cell line (4.5 million cells) and 5 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 30 million sequence tags were mapped to identify SAP30 binding sites. The image on the left shows SAP30 binding at transcriptional start sites across a 2.4 million bp region on chromosome 1. The image on the right is zoomed in to show SAP30 binding at two other start sites.)
Western Blot (WB)
(SAP30 antibody (pAb) tested by Western blot. Detection of SAP30 by Western blot analysis. Lane 1: Whole cell extract of A549 cells (20 ug). Lane 2: Nuclear extract of HeLa cells (20 ug). Both were probed with SAP30 antibody (pAb) at a 1:500 dilution.)
Western Blot (WB)
(SAP30 antibody (pAb) tested by Western blot. Detection of SAP30 by Western blot analysis. Lane 1: Whole cell extract of A549 cells (20 ug). Lane 2: Nuclear extract of HeLa cells (20 ug). Both were probed with SAP30 antibody (pAb) at a 1:500 dilution.)
Background: SAP30, also known as Sin3A-associated protein, 30kDa, is part of the SIN3 corepressor complex, that also includes SIN3, SAP18, HDAC1, HDAC2, RbAp46 and RbAp48. SAP30 is involved in the functional recruitment of the Sin3-histone deacetylase complex to a specific subset of N-CoR corepressor complexes.
NCBI and Uniprot Product Information
NCBI Description
Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP18, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This complex is active in deacetylating core histone octamers, but inactive in deacetylating nucleosomal histones. A pseudogene of this gene is located on chromosome 3. [provided by RefSeq, Jul 2008]
Uniprot Description
SAP30: Involved in the functional recruitment of the Sin3- histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes. Capable of transcription repression by N- CoR. Active in deacetylating core histone octamers (when in a complex) but inactive in deacetylating nucleosomal histones. Belongs to the SAP30 family.
Protein type: Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 4q34.1
Cellular Component: nucleoplasm; histone deacetylase complex
Molecular Function: protein binding; DNA binding; metal ion binding; transcription corepressor activity
Biological Process: establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; gene expression; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic
Research Articles on SAP30
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Product Notes
The SAP30 sap30 (Catalog #AAA388557) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SAP30 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SAP30 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Western Blot (WB). Applications Validated: ChIP: 5ul per ChIP ChIP-Seq & ChIP-chip: 5ul each WB: 1:500 dilution. Researchers should empirically determine the suitability of the SAP30 sap30 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAP30, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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