Rabbit anti-Human, Mouse SAP18 Polyclonal Antibody | anti-SAP18 antibody

SAP18 Antibody

Cat. Num. AAA9629813

• Gene Names: SAP18; SAP18P; 2HOR0202
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: SAP18; Polyclonal Antibody; SAP18 Antibody; 18 kDa Sin3-associated polypeptide; 2HOR0202; Cell growth-inhibiting gene 38 protein; Histone deacetylase complex subunit SAP18; SAP18_HUMAN; Sin3-associated polypeptide p18; anti-SAP18 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: SAP18 Antibody detects endogenous levels of SAP18.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-SAP18 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human SAP18.
• Conjugation: Unconjugated
• Similarity: Belongs to the SAP18 family.
• Subunit Structure: Found in a mRNA splicing-dependent exon junction complex (EJC). Component of the heterotrimeric ASAP (apoptosis- and splicing-associated protein) and PSAP complexes consisting of RNPS1, SAP18 and either ACIN1 or PNN, respectively; the ASAP and PSAP complexes probably are formed mutually exclusive. For the ASAP complex, the association of SAP18 seems to require a preformed RNPS1:ACIN1 complex. Forms a complex with SIN3A and HDAC1. Interacts with SUFU.
• Tissue Specificity: Ubiquitous.
• Function: Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP and PSAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. The ASAP complex can inhibit mRNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits the formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function.
• Note: huttles between the nucleus and the cytoplasm (PubMed:16314458). Colocalizes with ACIN1 and SRSF2 in nuclear speckles (PubMed:20966198).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-SAP18 antibody

Nucleus; Cytoplasm; Nucleus speckle; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 6831678
• NCBI GeneID: 10284
• UniProt Accession #: O00422; Q2TTR4; Q6IAW9; Q8N606; Q9UF14; B2R494
• Molecular Weight: 153
• NCBI Official Full Name: Histone deacetylase complex subunit SAP18
• NCBI Official Synonym Full Names: Sin3A-associated protein, 18kDa
• NCBI Official Symbol: SAP18
• NCBI Official Synonym Symbols: SAP18P; 2HOR0202
• NCBI Protein Information: histone deacetylase complex subunit SAP18; sin3-associated polypeptide p18; sin3-associated polypeptide, p18; cell growth inhibiting protein 38; cell growth-inhibiting protein 38; 18 kDa Sin3-associated polypeptide; sin3-associated polypeptide, 18 kDa; ce
• UniProt Protein Name: Histone deacetylase complex subunit SAP18
• Protein Family: Zinc finger A20 domain-containing stress-associated protein
• UniProt Gene Name: SAP18
• UniProt Entry Name: SAP18_HUMAN

NCBI Description

Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Dec 2008]

Uniprot Description

SAP18: Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Belongs to the SAP18 family.

Chromosomal Location of Human Ortholog: 13q12.11

Cellular Component: nucleoplasm; histone deacetylase complex; cytoplasm; nuclear speck

Molecular Function: protein binding; transcription corepressor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; negative regulation of nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; positive regulation of apoptosis; RNA splicing; gene expression; mRNA processing; regulation of gene expression, epigenetic; regulation of alternative nuclear mRNA splicing, via spliceosome