Western Blot (WB)
(Western blot analysis of SAP14 expression in HepG2 (A), RT4 (B) whole cell lysates.)
Rabbit SAP14 Polyclonal Antibody | anti-SF3B14 antibody
Anti-SAP14 Antibody
Western Blot (WB)
(Western blot analysis of SAP14 expression in HepG2 (A), RT4 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of SAP14 expression in HepG2 (A), RT4 (B) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of SAP14 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of SAP14 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site. [provided by RefSeq, Jul 2008]
Uniprot Description
SF3B6: subunit of the splicing factor SF3B. Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.
Protein type: Spliceosome; RNA splicing; RNA-binding
Chromosomal Location of Human Ortholog: 2p23
Cellular Component: nucleoplasm; U12-dependent spliceosome
Molecular Function: nucleotide binding
Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression
Research Articles on SF3B14
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Product Notes
The SF3B14 sf3b6 (Catalog #AAA821067) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SAP14 Antibody reacts with Human, Mouse, Rat, Bovine, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's SAP14 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC). WB (1/500 - 1/1000), IF/ICC (1/100 - 1/500). Researchers should empirically determine the suitability of the SF3B14 sf3b6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAP14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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