Human LILRA5/CD85f ELISA Kit | LILRA5 elisa kit

Human LILRA5/CD85f ELISA Kit

Cat. Num. AAA1753892

• Gene Names: LILRA5; CD85; LIR9; CD85F; ILT11; LIR-9; ILT-11; LILRB7
• Reactivity: Human
• Synonyms: LILRA5/CD85f; Human LILRA5/CD85f ELISA Kit; CD85; LIR9; CD85F; ILT11; LIR-9; ILT-11; LILRB7; leukocyte immunoglobulin like receptor A5; LILRA5 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human LILRA5

• Samples: cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 93.7pg/ml-6,000pg/ml
• Sensitivity: <10pg/ml
• Capture/Detection Antibodies: Capture antibody- a polyclonal antibody from goat|Detection antibody- a biotinylated polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|93.7|187.5|375|750|1500|3000|6000; O.D.|0.047|0.135|0.212|0.364|0.759|0.878|1.531|2.029
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|135|927|3289|br>Standard deviation|7.02|63.96|200.62|br>CV(%)|5.2%|6.9%|6.1%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|148|896|2885; Standard deviation7.99|71.68|222.14; CV(%)|5.4%|8%|7.7%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 135|164|158|154|153|8.57|5.6%; Sample 2: 927|981|964|939|953|60.99|6.4%; Sample 3: 3289|3116|3572|3284|3315|291.72|8.8%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: G42-R299
• Anticoagulant: heparin, EDTA or citrate
• Principle: Human LILRA5 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for LILRA5 has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: G42-R299) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LILRA5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LILRA5 amount of sample captured in plate.
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 6000pg/ml, 3000pg/ml, 1500pg/ml, 750pg/ml, 375pg/ml, 187.5pg/ml, 93.7pg/m human LILRA5 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" for details. It is recommended that each human LILRA5 standard solution and each sample be measured in duplicate.
• TMB Incubation Time: 25-30
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Testing Data

Related Product Information for LILRA5 elisa kit

The protein encoded by this gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family. LIR family members are known to have activating and inibitory functions in leukocytes. Crosslink of this receptor protein on the surface of monocytes has been shown to induce calcium flux and secretion of several proinflammatory cytokines, which suggests the roles of this protein in triggering innate immune responses. This gene is one of the leukocyte receptor genes that form a gene cluster on the chromosomal region 19q13. 4. Four alternatively spliced transcript variants encoding distinct isoforms have been described.

Product Categories/Family for LILRA5 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 32490553
• NCBI GeneID: 353514
• NCBI Accession #: NP_067073.1
• NCBI GenBank Nucleotide #: NM_021250.3
• UniProt Accession #: A6NI73; A6NHI3
• Molecular Weight: 31,538 Da
• NCBI Official Full Name: leukocyte immunoglobulin-like receptor subfamily A member 5 isoform 1
• NCBI Official Synonym Full Names: leukocyte immunoglobulin like receptor A5
• NCBI Official Symbol: LILRA5
• NCBI Official Synonym Symbols: CD85; LIR9; CD85F; ILT11; LIR-9; ILT-11; LILRB7
• NCBI Protein Information: leukocyte immunoglobulin-like receptor subfamily A member 5
• UniProt Protein Name: Leukocyte immunoglobulin-like receptor subfamily A member 5
• Protein Family: Leukocyte immunoglobulin-like receptor subfamily
• UniProt Gene Name: LILRA5
• UniProt Synonym Gene Names: ILT11; LILRB7; LIR9; ILT-11; LIR-9

NCBI Description

The protein encoded by this gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family. LIR family members are known to have activating and inibitory functions in leukocytes. Crosslink of this receptor protein on the surface of monocytes has been shown to induce calcium flux and secretion of several proinflammatory cytokines, which suggests the roles of this protein in triggering innate immune responses. This gene is one of the leukocyte receptor genes that form a gene cluster on the chromosomal region 19q13.4. Four alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

May play a role in triggering innate immune responses. Does not seem to play a role for any class I MHC antigen recognition.