Rabbit anti-Mouse SAG Polyclonal Antibody | anti-SAG antibody

SAG (S-arrestin, 48kD Protein, Retinal S-antigen, S-AG, Rod Photoreceptor Arrestin, SAG) (Biotin)

Cat. Num. AAA6459013

• Gene Names: SAG; RP47; S-AG
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SAG; Polyclonal Antibody; SAG (S-arrestin; 48kD Protein; Retinal S-antigen; S-AG; Rod Photoreceptor Arrestin; SAG) (Biotin); anti-SAG antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse SAG. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-SAG antibody: Western Blot (WB)
• Application Notes: WB: 1:2000; Applications are based on unconjugated antibody.
• Immunogen: KLH conjugated synthetic peptide corresponding to aa273-307 at the human SAG.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Categories/Family for anti-SAG antibody

Antibodies; Arrestin

References

Yamaki K., et al. FEBS Lett. 234:39-43(1988). Yamaki K., et al. FEBS Lett. 236:507-507(1988). Yamamoto S., et al. Nat. Genet. 15:175-178(1997). Hillier L.W., et al. Nature 434:724-731(2005). Roni V., et al. BMC Genomics 8:42-42(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 116008178
• NCBI GeneID: 6295
• NCBI Accession #: NP_000532.2
• NCBI GenBank Nucleotide #: NM_000541.4
• UniProt Accession #: P10523; Q53SV3; Q99858; A0FDN6
• Molecular Weight: 45,120 Da
• NCBI Official Full Name: S-arrestin
• NCBI Official Synonym Full Names: S-antigen; retina and pineal gland (arrestin)
• NCBI Official Symbol: SAG
• NCBI Official Synonym Symbols: RP47; S-AG
• NCBI Protein Information: S-arrestin
• UniProt Protein Name: S-arrestin
• Protein Family: S-arrestin
• UniProt Gene Name: SAG
• UniProt Synonym Gene Names: S-AG
• UniProt Entry Name: ARRS_HUMAN

NCBI Description

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. S-arrestin, also known as S-antigen, is a major soluble photoreceptor protein that is involved in desensitization of the photoactivated transduction cascade. It is expressed in the retina and the pineal gland and inhibits coupling of rhodopsin to transducin in vitro. Additionally, S-arrestin is highly antigenic, and is capable of inducing experimental autoimmune uveoretinitis. Mutations in this gene have been associated with Oguchi disease, a rare autosomal recessive form of night blindness. [provided by RefSeq, Jul 2008]

Uniprot Description

SAG: Arrestin is one of the major proteins of the ros (retinal rod outer segments); it binds to photoactivated- phosphorylated rhodopsin, thereby apparently preventing the transducin-mediated activation of phosphodiesterase. Retina and pineal gland. Belongs to the arrestin family.

Protein type: G protein regulator, misc.; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: cytosol; photoreceptor inner segment; photoreceptor outer segment

Molecular Function: opsin binding; phosphoprotein binding; protein phosphatase inhibitor activity

Biological Process: cell surface receptor linked signal transduction; negative regulation of catalytic activity; phototransduction, visible light; regulation of rhodopsin mediated signaling; rhodopsin mediated signaling; visual perception

Disease: Oguchi Disease 1; Retinitis Pigmentosa 47