Rabbit anti-Human SAE2 Polyclonal Antibody | anti-UBA2 antibody

SAE2, CT (SUMO-activating Enzyme Subunit 2, Anthracycline-associated Resistance ARX, Ubiquitin-like 1-activating Enzyme E1B, UBA2, UBLE1B, HRIHFB2115)

Cat. Num. AAA6000294

• Gene Names: UBA2; ARX; SAE2; HRIHFB2115
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: SAE2; Polyclonal Antibody; CT (SUMO-activating Enzyme Subunit 2; Anthracycline-associated Resistance ARX; Ubiquitin-like 1-activating Enzyme E1B; UBA2; UBLE1B; HRIHFB2115); Anti -SAE2; anti-UBA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human UBA2.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-UBA2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 0.25-0.5ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to human UBA2 at C-terminal conjugated to KLH.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of SAE2 in 293 cell lysate with SAE2 antibody at 0.25ug/ml in (A) the absence and (B) the presence of blocking peptide.)

Related Product Information for anti-UBA2 antibody

Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes. The conjugation of SUMO-1, SUMO-2 and SUMO-3 onto target proteins requires the concerted action of the specific E1-activating enzyme SAE1:SAE2, the E2-conjugating enzyme Ubc9, and an E3-like SUMO ligase.

Product Categories/Family for anti-UBA2 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 4885649
• NCBI GeneID: 10054
• NCBI Accession #: NP_005490.1
• NCBI GenBank Nucleotide #: NM_005499.2
• UniProt Accession #: Q9UBT2; O95605; Q59H87; Q6IBP6; Q9NTJ1; Q9UED2
• Molecular Weight: 71,224 Da
• NCBI Official Full Name: SUMO-activating enzyme subunit 2
• NCBI Official Synonym Full Names: ubiquitin-like modifier activating enzyme 2
• NCBI Official Symbol: UBA2
• NCBI Official Synonym Symbols: ARX; SAE2; HRIHFB2115
• NCBI Protein Information: SUMO-activating enzyme subunit 2; SUMO1 activating enzyme subunit 2; SUMO-1 activating enzyme subunit 2; ubiquitin-like 1-activating enzyme E1B; anthracycline-associated resistance ARX; UBA2, ubiquitin-activating enzyme E1 homolog
• UniProt Protein Name: SUMO-activating enzyme subunit 2
• Protein Family: SUMO-activating enzyme
• UniProt Gene Name: UBA2
• UniProt Synonym Gene Names: SAE2; UBLE1B
• UniProt Entry Name: SAE2_HUMAN

NCBI Description

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]

Uniprot Description

SAE2: a protein of the ubiquitin-activating E1 family. Acts as a UBL1 E1 ligase. Mediates ATP-dependent activation of UBL1 and formation of a thiolester with a conserved cysteine residue on SAE2.

Protein type: EC 6.3.2.-; Ubiquitin ligase; Ligase; EC 6.3.2.19; Motility/polarity/chemotaxis; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: nucleoplasm; cytosol; nucleus

Molecular Function: SUMO activating enzyme activity; protein binding; protein heterodimerization activity; metal ion binding; enzyme activator activity; transcription factor binding; ATP binding

Biological Process: positive regulation of catalytic activity; cellular protein metabolic process; protein sumoylation; SMT3-dependent protein catabolic process; post-translational protein modification