Rabbit anti-Human S100A1 Polyclonal Antibody | anti-S100A1 antibody

S100A1, CT (S100A1, S100A, Protein S100-A1, S-100 protein alpha chain, S-100 protein subunit alpha, S100 calcium-binding protein A1) (PE)

Cat. Num. AAA6344622

• Gene Names: S100A1; S100; S100A; S100-alpha
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: S100A1; Polyclonal Antibody; CT (S100A1; S100A; Protein S100-A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 calcium-binding protein A1) (PE); S100 calcium-binding protein A1; anti-S100A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-S100A1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: S100A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-83 amino acids from the C-terminal region of human S100A1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(S100A1 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the S100A1 antibody detected the S100A1 protein (arrow).)

Immunohistochemistry (IHC)

(S100A1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of S100A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-S100A1 antibody

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies.

Product Categories/Family for anti-S100A1 antibody

Antibodies; Binding Proteins; Calcium

References

Marlatt, N.M., et al. Protein Expr. Purif. 73(1):58-64(2010). van Dieck, J., et al. FEBS Lett. 584(15):3269-3274(2010). Sviatoha, V., et al. Melanoma Res. 20(2):118-125(2010). DeRycke, M.S., et al. Am. J. Clin. Pathol. 132(6):846-856(2009). Yusenko, M.V., et al. Int. J. Biol. Sci. 5(6):517-527(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 5454032
• NCBI GeneID: 6271
• NCBI Accession #: NP_006262.1
• NCBI GenBank Nucleotide #: NM_006271.1
• UniProt Accession #: P23297; Q5T7Y3; B2R5D9
• Molecular Weight: 10,546 Da
• NCBI Official Full Name: protein S100-A1
• NCBI Official Synonym Full Names: S100 calcium binding protein A1
• NCBI Official Symbol: S100A1
• NCBI Official Synonym Symbols: S100; S100A; S100-alpha
• NCBI Protein Information: protein S100-A1; S100 alpha; S-100 protein alpha chain; S-100 protein subunit alpha; S100 calcium-binding protein A1; S100 protein, alpha polypeptide
• UniProt Protein Name: Protein S100-A1
• Protein Family: Protein
• UniProt Gene Name: S100A1
• UniProt Synonym Gene Names: S100A
• UniProt Entry Name: S10A1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. [provided by RefSeq, Jul 2008]

Uniprot Description

S100A1: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. Interacts with AGER and CAPZA1. Interacts with FKBP4. Interacts with RYR1 and RYR2. Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. Belongs to the S-100 family.

Protein type: Calcium-binding; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: neuron projection; protein complex; sarcoplasmic reticulum; M band; Z disc; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; calcium ion binding; calcium-dependent protein binding; ATPase binding

Biological Process: substantia nigra development; negative regulation of transcription from RNA polymerase II promoter; regulation of heart contraction