Mouse anti-Human Prolactin Monoclonal Antibody | anti-PRL antibody

Mouse anti Human Prolactin:Biotin

Cat. Num. AAA226224

• Reactivity: Human
• Applications: ELISA
• Purity: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Synonyms: Prolactin; Monoclonal Antibody; Mouse anti Human Prolactin:Biotin; Prolactin antibody | C03-3E9; PROLACTIN; anti-PRL antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: C03-3E9
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Form/Format: Phosphate buffered saline; 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin; Purified IgG conjugated to Biotin - liquid
• Concentration: IgG concentration 0.1mg/ml (varies by lot)

• Applicable Applications for anti-PRL antibody: ELISA (EIA)
• Application Notes: ELISA: 1.0ug/ml
• Immunogen: E Coli derived recombinant human prolactin (Leu29-Cys227)
• Fusion Partners: Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
• Conjugation: Biotin
• Preparation and Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.; ; Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

ELISA (EIA)

(Sandwich ELISA analysis of human prolactin using Mouse anti Human prolactin as a capture reagent and biotinylated Mouse anti Human prolactin (MBS226224) as a detection reagent with recombinant human prolactin as the antigen. Detection was perfo)

Related Product Information for anti-PRL antibody

Mouse anti Human prolactin antibody, clone C03-3E9, recognizes prolactin a peptide hormone expressed by many cell types including the pituitary gland, decidua, ovary, prostate, mammary gland, adipose tissue, brain and immune cells. Of the immune cells, prolactin is secreted by dentritic cells, thymocytes, T cells, B cells, monocytes and macrophages (Borba et al. 2018). Prolactin now has a reported >300 separate biological activities, since it was first discovered as a stimulator of crop milk in pigeons and a promotor of lactation in rabbits (Freeman et al. 2000). In the immune system prolactin acts as a cytokine. In an example, prolactin is able to regulate innate and adaptive immune responses through the maturation of CD4- CD8- thymocytes to CD4+ CD8+ T cells via IL-2 receptor expression. Prolactin is also able to stimulate the development of antigen presenting cells expressing major histocompatibility complex class II (Borba et al. 2018). Where patients have an abnormally high level of circulating prolactin (hyperprolactinemia) this can result in the impairment of B cell clonal deletion, deregulation or receptor editing, as well as the diminishment of the threshold for B cell activation, promoting auto-reactivity (Borba et al. 2018).

The biotinylated Mouse anti Human prolactin antibody, clone C03-3E9 can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human NGAL antibody, clone C01-2B2 as the capture antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 254675133
• NCBI GeneID: 5617
• NCBI Accession #: NP_001157030.1
• NCBI GenBank Nucleotide #: NM_001163558.2
• UniProt Accession #: P01236; Q15199; Q92996
• Molecular Weight: 25,876 Da
• NCBI Official Full Name: prolactin
• NCBI Official Synonym Full Names: prolactin
• NCBI Official Symbol: PRL
• NCBI Protein Information: prolactin; decidual prolactin
• UniProt Protein Name: Prolactin
• Protein Family: Prolactin
• UniProt Gene Name: PRL
• UniProt Synonym Gene Names: PRL
• UniProt Entry Name: PRL_HUMAN

NCBI Description

This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]

Uniprot Description

prolactin: Prolactin acts primarily on the mammary gland by promoting lactation. Belongs to the somatotropin/prolactin family.

Protein type: Secreted; Cytokine; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: extracellular region

Molecular Function: protein binding; prolactin receptor binding; hormone activity

Biological Process: lactation; cell proliferation; cell surface receptor linked signal transduction; regulation of multicellular organism growth; positive regulation of JAK-STAT cascade; female pregnancy