Goat Ryk Polyclonal Antibody | anti-Ryk antibody
Goat anti-Ryk (mouse) Antibody
Gene Names
Ryk; Vik; ERK-3; AW536699
Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Ryk; Polyclonal Antibody; Goat anti-Ryk (mouse) Antibody; receptor-like tyrosine kinase; AW536699; ERK-3; Vik; NYK-R; growth factor receptor; kinase VIK; met-related kinase; tyrosine-protein kinase RYK; AW536699 antibody; ERK-3 antibody; growth factor receptor antibody; kinase VIK antibody; met-related kinase antibody; NYK-R antibody; receptor-like tyrosine kinase antibody; Ryk antibody; tyrosine-protein kinase RYK antibody; Vik antibody; Ryk (mouse); anti-Ryk antibody
Host
Goat
Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
HAKSKVEYKLGFQ
Sequence Length
594
Applicable Applications for anti-Ryk antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Preliminary experiments gave an approx 85kDa band in Mouse Lung lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 66.3kDa according to NP_038677.3. The 85kDa band was successfully blocked by incubation with the immunizing peptide.
Western Blot: Preliminary experiments gave an approx 85kDa band in Mouse Lung lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 66.3kDa according to NP_038677.3. The 85kDa band was successfully blocked by incubation with the immunizing peptide.
Immunogen
Peptide with sequence C-HAKSKVEYKLGFQ, from the internal region of the protein sequence according to NP_038677.3; NP_001036072.1.
Epitope
Internal region
Note
This antibody is expected to recognize both reported isoforms (NP_038677.3; NP_001036072.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,285 Da
NCBI Official Full Name
tyrosine-protein kinase RYK isoform 1
NCBI Official Synonym Full Names
receptor-like tyrosine kinase
NCBI Official Symbol
Ryk
NCBI Official Synonym Symbols
Vik; ERK-3; AW536699
NCBI Protein Information
tyrosine-protein kinase RYK
UniProt Protein Name
Tyrosine-protein kinase RYK
Protein Family
UniProt Gene Name
Ryk
UniProt Synonym Gene Names
Mrk
UniProt Entry Name
RYK_MOUSE
Uniprot Description
RYK: a receptor tyrosine kinase of the Ryk family. Associates with, and may modulate the signaling of, EphB2 and EphB3. Expressed in nestin-positive progenitor cells and neurons, some oligodendrocytes, O-2A progenitor cells, and type-2 astrocytes in developing CNS.
Protein type: Protein kinase, TK; Protein kinase, tyrosine (receptor); Kinase, protein; Membrane protein, integral; EC 2.7.10.1; TK group; Ryk family
Cellular Component: cytoplasm; integral to membrane; membrane; nucleus; plasma membrane
Molecular Function: ATP binding; frizzled binding; kinase activity; nucleotide binding; protein binding; protein kinase activity; protein-tyrosine kinase activity; transferase activity; transmembrane receptor activity; transmembrane receptor protein tyrosine kinase activity; Wnt receptor activity; Wnt-protein binding
Biological Process: axon guidance; axonogenesis; corpus callosum development; negative chemotaxis; negative regulation of axon extension involved in axon guidance; neurite development; neuron differentiation; phosphorylation; positive regulation of MAPKKK cascade; protein amino acid phosphorylation; skeletal morphogenesis; Wnt receptor signaling pathway
Research Articles on Ryk
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Product Notes
The Ryk ryk (Catalog #AAA423143) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Ryk (mouse) Antibody reacts with Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Ryk can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Preliminary experiments gave an approx 85kDa band in Mouse Lung lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 66.3kDa according to NP_038677.3. The 85kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the Ryk ryk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HAKSKVEYKL GFQ. It is sometimes possible for the material contained within the vial of "Ryk, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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