Rabbit RUFY3 Polyclonal Antibody | anti-RUFY3 antibody
RUFY3 Antibody
Gene Names
RUFY3; RIPX; SINGAR1; ZFYVE30
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
RUFY3; Polyclonal Antibody; RUFY3 Antibody; Protein RUFY3; rap2 interacting protein x; Rap2 interacting protein x isoform 2; Rap2-interacting protein x; RIPx; Rpipx; Rufy3; RUFY3_HUMAN; RUN and FYVE domain containing 3; Singar; Singar1; Single axon regulated protein; single axon related 1; Single axon-regulated protein; anti-RUFY3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
RUFY3 Antibody detects endogenous levels of RUFY3.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-RUFY3 antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human RUFY3.
Conjugation
Unconjugated
Post Translational Modifications
Phosphorylated by PAK1. Isoform 1 is partially phosphorylated (By similarity).
Subunit Structure
Interacts with PAK1. Interacts (via C-terminus) with Ras-related Rab-5 proteins. Interacts (via C-terminus) with Ras-related Rap-2 proteins. Interacts with PIK3CA and PIK3R1 (By similarity). Interacts (via N-terminus) with FSCN1; this interaction induces neuron axon development (By similarity). Interacts with DBN1 (By similarity).
Tissue Specificity
Overexpressed in gastric cancer cells and tissues (at protein level).
Function
Plays a role in the generation of neuronal polarity formation and axon growth (By similarity). Implicated in the formation of a single axon by developing neurons (By similarity). May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes (By similarity). Plays a role in the formation of F-actin-enriched protrusive structures at the cell periphery. Plays a role in cytoskeletal organization by regulating the subcellular localization of FSCN1 and DBN1 at axonal growth cones (By similarity). Promotes gastric cancer cell migration and invasion in a PAK1-dependent manner.
Note
Colocalizes with PAK1, F-actin, myosins and integrins in invadopodia at the cell periphery (PubMed:25766321). Colocalized with Ras-related Rab-5 proteins in cytoplasmic vesicles (PubMed:20376209). Accumulates in axon growth cones in a F-actin-dependent manner (By similarity). Colocalized with FSCN1 and F-actin at filipodia and lamellipodia of axonal growth cones (By similarity). Colocalized with DBN1 and F-actin at transitional domain of the axonal growth cone (By similarity).
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Product Categories/Family for anti-RUFY3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,721 Da
NCBI Official Full Name
protein RUFY3 isoform 1
NCBI Official Synonym Full Names
RUN and FYVE domain containing 3
NCBI Official Symbol
RUFY3
NCBI Official Synonym Symbols
RIPX; SINGAR1; ZFYVE30
NCBI Protein Information
protein RUFY3; rap2 interacting protein x; single axon-regulated protein; single axon-related 1
UniProt Protein Name
Protein RUFY3
Protein Family
UniProt Gene Name
RUFY3
UniProt Synonym Gene Names
KIAA0871; RIPx; Singar
UniProt Entry Name
RUFY3_HUMAN
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Product Notes
The RUFY3 rufy3 (Catalog #AAA9630786) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RUFY3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RUFY3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the RUFY3 rufy3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RUFY3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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