Mouse g-Catenin (C-terminal) Monoclonal Antibody

Anti-gamma-Catenin (C-terminal)

Cat. Num. AAA470014

• Reactivity: Human, Rat, Mouse
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry
• Purity: Protein A Purified
• Synonyms: g-Catenin (C-terminal); Monoclonal Antibody; Anti-gamma-Catenin (C-terminal); Junction Plakoglobin; JUP; Desmoplakin III; Desmoplakin-3; anti-g-Catenin (C-terminal) antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Specificity: The antibody detects an 84kDa* protein corresponding to the molecular mass of gamma-Catenin on SDS-PAGE immunoblots of A431 and Hct116 src transformed cells. In addition, this antibody recognizes only gamma-Catenin in immunoprecipitations using anti-gamma-Catenin versus anti- beta -Catenin.
• Purity/Purification: Protein A Purified
• Form/Format: Mouse monoclonal antibody purified with protein A chromatography is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-g-Catenin (C-terminal) antibody: ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry
• Application Notes: ELISA: 1:2000; IP: 1:100; WB: 1:1000; ICC: 1:50; ; End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
• Immunogen: Clone was generated from a recombinant protein that includes amino acid residues in the C-terminal region of rat gamma-Catenin. This peptide sequence is highly conserved in human and mouse gamma-Catenin.
• Preparation and Storage: Store at -20°C. Stable for 1 year.

Testing Data

(Western blot analysis of anti-gamma-Catenin (C-terminal) immunoprecipitates from pervanadate-treated A431. The immunoprecipitates were untreated (lanes 1,3,7) or treated with alkaline phosphatase (lanes 2,4,8). The blots were probed with gamma-Catenin (a.a. 545-555), gamna-Catenin (Tyr-550) or gamma-Catenin (C-terminal) antibodies. The anti-gamma-Catenin (Tyr-550) was used in the presence of gamma-Catenin (Tyr-550) (lane 5) or gamma-Catenin (Tyr-644) (lane 6) peptides.)

IHC

( Immunocytochemical labeling of phosphorylated gamma-Catenin in control (Top) and pervanadate-treated (Bottom) A431 cells. The cells were co-labeled with mouse monoclonal gamma-Catenin or rabbit polyclonal gamma-Catenin (Tyr-550) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy2 or Cy3.)

Related Product Information for anti-g-Catenin (C-terminal) antibody

Plakoglobin (gamma-Catenin) is a catenin family member identified as a component of desmosomes. gamma -Catenin has high homology to beta-catenin and, like beta-catenin, it can associate with the cadherins, E-cadherin and N-cadherin. One molecule of alpha-catenin and at least one molecule of beta-catenin and gamma -Catenin simultaneously bind to a single cadherin molecule. A 19-amino acid sequence of desmoglein was found to be critical for binding of gamma -Catenin. Similar catenin-binding domains found in cadherins, suggest a common mechanism for gamma -Catenin localization to both adherens junctions and desmosomes. Phosphorylation of tyrosine residues in gamma -Catenin can modify its interactions with other proteins. Phosphorylation of tyrosine 644 decreases gamma -Catenin association with alpha-catenin, but increases binding to desmoplakin. Fer kinase can phosphorylate tyrosine 550, which increases gamma -Catenin binding to ?-catenin. Thus, tyrosine phosphorylation may be important for regulation of gamma -Catenin protein-protein interactions within desmosomal complexes.

References

• McCrea, P.D. et al. (1991) Science 254:1359.
• Miravet, S. et al. (2003) Mol. Cell. Biol. 23(20) :7391.