Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded rat cerebellum (Fig. 1 & 2) and rat hippocampus (Fig. 3) tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:300 dilution of G3500-12D Reactivity was detected using the IHC-Select Detection Kit Staining is observed in the cytoplasm of Purkinje cells but not in the nucleus (granular layer junction) in Figures 1 & 2. In Fig. 3, the staining is seen in the cytoplasm of cells in area A3 of hippocampus.)
Mouse anti-Human, Rat GluR2 Monoclonal Antibody | anti-GluR2 antibody
GluR2 (Glutamate Receptor) (MaxLight 650)
Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded rat cerebellum (Fig. 1 & 2) and rat hippocampus (Fig. 3) tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:300 dilution of G3500-12D Reactivity was detected using the IHC-Select Detection Kit Staining is observed in the cytoplasm of Purkinje cells but not in the nucleus (granular layer junction) in Figures 1 & 2. In Fig. 3, the staining is seen in the cytoplasm of cells in area A3 of hippocampus.)
Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded rat cerebellum (Fig. 1 & 2) and rat hippocampus (Fig. 3) tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:300 dilution of G3500-12D Reactivity was detected using the IHC-Select Detection Kit Staining is observed in the cytoplasm of Purkinje cells but not in the nucleus (granular layer junction) in Figures 1 & 2. In Fig. 3, the staining is seen in the cytoplasm of cells in area A3 of hippocampus.)
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. Rat brain membrane tissue lysate was probed with G3500-12D. Proteins were visualized using a Goat Anti-Mouse IgG secondary antibody conjugated to HRP and chemiluminescence detection system. Arrow indicates GluR2 (~ 96 kDa).)
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. Rat brain membrane tissue lysate was probed with G3500-12D. Proteins were visualized using a Goat Anti-Mouse IgG secondary antibody conjugated to HRP and chemiluminescence detection system. Arrow indicates GluR2 (~ 96 kDa).)
NCBI and Uniprot Product Information
NCBI Description
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, GRIA1-4. The subunit encoded by this gene (GRIA2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Human and animal studies suggest that pre-mRNA editing is essential for brain function, and defective GRIA2 RNA editing at the Q/R site may be relevant to amyotrophic lateral sclerosis (ALS) etiology. Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
GluR2: an integral membrane protein belonging to the glutamate-gated ion channel family. L-glutamate (Glu) acts as an excitatory neurotransmitter at many synapses in the central nervous system. Glutamate receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA(quisqualate) > glutamate > kainate. Each of the four GluR proteins (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing.
Protein type: Membrane protein, multi-pass; Channel, ligand-gated; Channel, calcium; Membrane protein, integral
Chromosomal Location of Human Ortholog: 4q32.1
Cellular Component: postsynaptic membrane; synaptic vesicle; endoplasmic reticulum membrane; integral to plasma membrane; dendrite; plasma membrane; cell junction
Molecular Function: protein binding; extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity
Biological Process: synaptic transmission; synaptic transmission, glutamatergic; ionotropic glutamate receptor signaling pathway; signal transduction
Research Articles on GluR2
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Product Notes
The GluR2 gria2 (Catalog #AAA6246768) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GluR2 (Glutamate Receptor) (MaxLight 650) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GluR2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the GluR2 gria2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GluR2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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