Rabbit anti-Human RTF1 Polyclonal Antibody | anti-RTF1 antibody

RTF1 Antibody

Cat. Num. AAA7046618

• Gene Names: RTF1; GTL7; KIAA0252
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: RTF1; Polyclonal Antibody; RTF1 Antibody; RNA polymerase-associated protein RTF1 homolog; KIAA0252; anti-RTF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 710

• Applicable Applications for anti-RTF1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Species: Human
• Immunogen: Recombinant human RNA polymerase-associated protein RTF1 homolog protein
• Conjugate: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate cancer using MBS7046618 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human cervical cancer using MBS7046618 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of Hela cells using MBS7046618 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Western Blot (WB)

(Western BlotPositive WB detected in:Hela whole cell lysate,HEK293 whole cell lysate,MCF-7 whole cell lysate,HepG2 whole cell lysateAll lanes: RTF1 antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 81 kDaObserved band size: 81 kDa)

Related Product Information for anti-RTF1 antibody

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Binds single-stranded DNA. Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 195976782
• NCBI GeneID: 23168
• NCBI Accession #: NP_055953.3
• NCBI GenBank Nucleotide #: NM_015138.4
• UniProt Accession #: Q92541; Q96BX6
• Molecular Weight: 80,313 Da
• NCBI Official Full Name: RNA polymerase-associated protein RTF1 homolog
• NCBI Official Synonym Full Names: RTF1 homolog, Paf1/RNA polymerase II complex component
• NCBI Official Symbol: RTF1
• NCBI Official Synonym Symbols: GTL7; KIAA0252
• NCBI Protein Information: RNA polymerase-associated protein RTF1 homolog
• UniProt Protein Name: RNA polymerase-associated protein RTF1 homolog
• Protein Family: Replication termination factor
• UniProt Gene Name: RTF1
• UniProt Synonym Gene Names: KIAA0252
• UniProt Entry Name: RTF1_HUMAN

NCBI Description

This locus may represent a gene involved in regulation of transcription elongation and chromatin remodeling, based on studies of similar proteins in other organisms. The encoded protein may bind single-stranded DNA. [provided by RefSeq, Sep 2010]

Uniprot Description

RTF1: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1; it promotes leukemogenesis though association with MLL-rearranged oncoproteins, such as MLL-MLLT3/AF9 and MLL-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Binds single- stranded DNA. Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 15q15.1

Molecular Function: protein binding; single-stranded DNA binding

Biological Process: endodermal cell fate commitment; negative regulation of transcription from RNA polymerase II promoter; positive regulation of RNA elongation from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; stem cell maintenance

Disease: Congenital Disorder Of Glycosylation, Type In