Rabbit anti-Human RTF1 Polyclonal Antibody | anti-RTF1 antibody

RTF1, ID (RTF1, KIAA0252, RNA polymerase-associated protein RTF1 homolog) (Biotin)

Cat. Num. AAA6344317

• Gene Names: RTF1; GTL7; KIAA0252
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RTF1; Polyclonal Antibody; ID (RTF1; KIAA0252; RNA polymerase-associated protein RTF1 homolog) (Biotin); RNA polymerase-associated protein RTF1 homolog; anti-RTF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RTF1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RTF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 441-470 amino acids from the Central region of human RTF1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RTF1 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the RTF1 antibody detected the RTF1 protein (arrow).)

Related Product Information for anti-RTF1 antibody

This locus may represent a gene involved in regulation of transcription elongation and chromatin remodeling, based on studies of similar proteins in other organisms. The encoded protein may bind single-stranded DNA.

Product Categories/Family for anti-RTF1 antibody

Antibodies; KIAA; orf

References

de Jong, R.N., et al. Structure 16(1):149-159(2008). Warner, M.H., et al. Mol. Cell. Biol. 27(17):6103-6115(2007). Rozenblatt-Rosen, O., et al. Mol. Cell. Biol. 25(2):612-620(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 195976782
• NCBI GeneID: 23168
• NCBI Accession #: NP_055953.3
• NCBI GenBank Nucleotide #: NM_015138.4
• UniProt Accession #: Q92541; Q96BX6
• Molecular Weight: 80,313 Da
• NCBI Official Full Name: RNA polymerase-associated protein RTF1 homolog
• NCBI Official Synonym Full Names: Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• NCBI Official Symbol: RTF1
• NCBI Official Synonym Symbols: GTL7; KIAA0252
• NCBI Protein Information: RNA polymerase-associated protein RTF1 homolog; ortholog of mouse gene trap locus 7
• UniProt Protein Name: RNA polymerase-associated protein RTF1 homolog
• Protein Family: Replication termination factor
• UniProt Gene Name: RTF1
• UniProt Synonym Gene Names: KIAA0252
• UniProt Entry Name: RTF1_HUMAN

Uniprot Description

RTF1: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1; it promotes leukemogenesis though association with MLL-rearranged oncoproteins, such as MLL-MLLT3/AF9 and MLL-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Binds single- stranded DNA. Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 15q15.1

Cellular Component: nucleolus

Molecular Function: protein binding; single-stranded DNA binding

Biological Process: Wnt receptor signaling pathway; endodermal cell fate commitment; positive regulation of RNA elongation from RNA polymerase II promoter; stem cell maintenance; transcription initiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation

Disease: Congenital Disorder Of Glycosylation, Type In