Rabbit PCB Polyclonal Antibody | anti-PCB antibody

PCB Polyclonal Antibody

Cat. Num. AAA3006066

• Gene Names: PC; PCB
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PCB; Polyclonal Antibody; PCB Polyclonal Antibody; Pyruvate carboxylase; mitochondrial; Pyruvic carboxylase; PC; anti-PCB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PCB polyclonal antibody detects endogenous levels of PCB protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1178

• Applicable Applications for anti-PCB antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 400-445 of Human PCB.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PCB polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:NIH-T3 cell lysateLane3:H9C2 cell lyate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PCB pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-PCB antibody

Pyruvate carboxylase (PCB) is a biotinylated mitchondrial enzyme that catalyzes the synthesis of oxaloacetate from pyruvate in a tissue specific manner. In addition to covalently binding the biotin cofactor, PCB contains consensus sequences for the attachment of ATP and the substrate pyruvate. The PCB gene is located on the long arm of chromosome 11. Mutations in PCB metabolism (pyruvate carboxylase deficiency) are known to cause lactic acidosis, hypoglycemia and mental retardation.

NCBI and Uniprot Product Information

• NCBI GI #: 106049295
• NCBI GeneID: 5091
• NCBI Accession #: NP_000911.2
• NCBI GenBank Nucleotide #: NP_000911.2
• UniProt Accession #: P11498
• Molecular Weight: ~ 130kDa
• NCBI Official Full Name: pyruvate carboxylase, mitochondrial
• NCBI Official Synonym Full Names: pyruvate carboxylase
• NCBI Official Symbol: PC
• NCBI Official Synonym Symbols: PCB
• NCBI Protein Information: pyruvate carboxylase, mitochondrial
• UniProt Protein Name: Pyruvate carboxylase, mitochondrial
• Protein Family: Chlorophyll a/b light-harvesting protein
• UniProt Gene Name: PC
• UniProt Synonym Gene Names: PCB
• UniProt Entry Name: PYC_HUMAN

NCBI Description

This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PC: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate. Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency). PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.

Protein type: Carbohydrate Metabolism - pyruvate; Ligase; Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial; EC 6.4.1.1

Chromosomal Location of Human Ortholog: 11q13.4-q13.5

Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; mitochondrial inner membrane; cytosol

Molecular Function: pyruvate carboxylase activity; protein binding; DNA binding; metal ion binding; biotin carboxylase activity; biotin binding; ATP binding

Biological Process: oxaloacetate metabolic process; vitamin metabolic process; viral RNA genome packaging; carbohydrate metabolic process; glucose metabolic process; pathogenesis; lipid metabolic process; pyruvate metabolic process; water-soluble vitamin metabolic process; biotin metabolic process; gluconeogenesis

Disease: Pyruvate Carboxylase Deficiency