Rabbit RSV (B1) glycoprotein G/RSV-G Polyclonal Antibody | anti-RSV-G antibody

Human RSV (B1) glycoprotein G/RSV-G Antibody, Rabbit PAb, Antigen Affinity Purified

• Reactivity: RSV
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: RSV (B1) glycoprotein G/RSV-G; Polyclonal Antibody; Human RSV (B1) glycoprotein G/RSV-G Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-G Antibody; anti-RSV-G antibody

• Host: Rabbit
• Reactivity: RSV
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human RSV (B1) glycoprotein G/RSV-G
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid. 0.2um filtered solution in PBS

• Applicable Applications for anti-RSV-G antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-1:5000; ELISA: 1:5000-1:10,000; Antibody's applications haven't been validated with corresponding virus positive samples.
• Immunogen: Recombinant Human RSV (B1) glycoprotein G/RSV-G Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human RSV (B1) glycoprotein G/RSV-G. Human RSV (B1) glycoprotein G/RSV-G specific IgG was purified by Human RSV (B1) glycoprotein G/RSV-G affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Western Blot (WB)

(Anti-Human RSV (B1) glycoprotein G / RSV-G rabbit polyclonal antibody at 1:1000 dilution.Sample:Human RSV (B1) glycoprotein G / RSV-G Recombinant ProteinLane A: 20ngLane B: 5ngSecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.)

Related Product Information for anti-RSV-G antibody

Background: Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. HRSV G protein is a type II glycoprotein of 289-299 amino acids (depending on the virus strain) with a signal/anchor hydrophobic domain and is extensively modified by the addition of both N-and O-linked oligosaccharides to achieve the mature form of 8-9kDa. The C-terminal ectodomain of the G protein has a central region and four cysteines which are conserved in all HRSV isolates and have been proposed as the putative receptor binding site. The G protein mediates attachment of the virus to the host cell membrane by interacting with heparan sulfate, initiating the infection. As similar to mucins in amino acid compositions, the RSV G protein can interact with host CX3CR1, the receptor for the CX3C chemokine fractalkine, and thus modulates the immune response and facilitate infection. Secreted glycoprotein G helps RSV escape antibody-dependent restriction of replication by acting as an antigen decoy and by modulating the activity of leukocytes bearing Fcgamma receptors. Unlike the other paramyxovirus attachment proteins, HRSV-G lacks both neuraminidase and hemagglutinating activities.

References

Martin-Gallardo A. et al., 1993, J Gen Virol. 74 : 453-8. Jose AM. et al.,1997, J Gen Virol. 78: 2411-8. Feldman SA. et al., 1999, J Virol. 73: 6610-7. Garca-a-Beato R. et al., 2000, J Gen Virol. 81: 919-27. Zlateva KT. et al., 2004, J Virol. 78: 4675-83. Trento A. et al., 2006, J Virol. 80: 975-84.