Rabbit Influenza A H7N7 Neuraminidase/NA Polyclonal Antibody | anti-NA antibody

Influenza A H7N7 (A/Netherlands/219/2003) Neuraminidase/NA Antibody, Rabbit PAb, Antigen Affinity Purified

Cat. Num. AAA8110627

• Reactivity: H7N7
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: Influenza A H7N7 Neuraminidase/NA; Polyclonal Antibody; Influenza A H7N7 (A/Netherlands/219/2003) Neuraminidase/NA Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-NA Antibody; Anti-Neuraminidase Antibody; anti-NA antibody

• Host: Rabbit
• Reactivity: H7N7
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Influenza A H7N7 (A/Netherlands/219/2003) Neuraminidase/NA
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NA antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-1:5000; ELISA: 1:5000-1:10,000; Antibody's applications haven't been validated with corresponding virus positive samples.
• Immunogen: Recombinant Influenza A H7N7 (A/Netherlands/219/2003) Neuraminidase/NA Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Influenza A H7N7 (A/Netherlands/219/2003) Neuraminidase/NA. Influenza A H7N7 (A/Netherlands/219/2003) Neuraminidase/NA specific IgG was purified by Influenza A H7N7 (A/Netherlands/219/2003) Neuraminidase/NA affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-Influenza A H7N7 (A/Netherlands/219/2003) Neuraminidase / NA rabbit polyclonal antibody at 1:1000 dilution.Sample:Influenza A H7N7 (A/Netherlands/219/2003) Neuraminidase / NA Recombinant ProteinLane A: 5ngLane B: 1ngSecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.)

Related Product Information for anti-NA antibody

Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza identification, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early identification of influenza viral infection. MyBioSource has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.

References

Sardet C., et al., (1989), Molecular cloning, primary structure, and expression of the human growth factor-activatable Na+/H+ antiporter. Cell 56:271-280.Sardet C., et al., (1990), Growth factors induce phosphorylation of the Na+/H+ antiporter, glycoprotein of 110 kD.Science 247:723-726.Tse C.-M., et al., (1991), Molecular cloning and expression of a cDNA encoding the rabbit ileal villus cell basolateral membrane Na+/H+ exchanger.EMBO J. 10:1957-1967.

NCBI and Uniprot Product Information

• NCBI GI #: 38112638
• Molecular Weight: 51,806 Da
• NCBI Official Full Name: neuraminidase
• Protein Family: Neuraminidase