Sheep anti-Human RSPO-3 Polyclonal Antibody | anti-RSPO1 antibody

RSPO-3 (R-Spondin 3, Cristin-1)

Cat. Num. AAA6003129

• Gene Names: RSPO1; RSPO; CRISTIN3; RP11-566C13.1
• Reactivity: Human
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: RSPO-3; Polyclonal Antibody; RSPO-3 (R-Spondin 3; Cristin-1); Anti -RSPO-3 (R-Spondin 3; anti-RSPO1 antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human R-Spondin 3. Species sequence homology: recombinant mouse R-Spondin 3 (1%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.

• Application Notes: Suitable for use in Neutralization.
• Immunogen: Recombinant corresponding to aa22-272 from mouse R-Spondin expressed in CHO cells (Q9BXY4).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-RSPO1 antibody

R-Spondin 3 (RSPO3, roof plate-specific spondin 3), also called cysteine-rich and single thrombospondin domain containing-1 (Cristin 1), is an ~31kD secreted protein that shares ~40aa identity with the other three R-Spondin family members (1, 2). All are positive modulators of Wnt/b-catenin signaling, but each has a distinct expression pattern (1-4). Like other R-spondins, R-Spondin 3 contains two adjacent cysteine-rich furin-like domains (aa35-135) with one potential N-glycosylation site (aa36), followed by a thrombospondin (TSP-1) motif (aa147-207) and a region rich in basic residues (aa211-269). Only the furin-like domains are needed for b-catenin stabilization (2). Within aa21-209, human R-Spondin 3 shares 93%, 92%, 97%, 96% and 92aa identity with mouse, rat, equine, bovine and canine R-Spondin 3, respectively. Potential isoforms of 279-297aa diverge at aa210-276, respectively (5). Mouse R-Spondin 3 is critical for development of the placental labyrinthine layer, probably by promoting VEGF expression and thus vascular development (6, 7). It is also essential for expression of the placenta- specific transcription factor, Gcm1. In the mouse embryo, R-Spondin 3 is often expressed by or located near endothelial cells (6). It is found in the roof plate, tail, somites, otic vesicles, cephalic mesoderm, truncus arteriosus, atrioventricular canal of the developing heart, and strongly but transiently in developing limbs (4, 7). R-Spondins regulate Wnt/b-catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK-1- mediated internalization (8, 9). Reports differ on whether R-Spondins bind LRP-6 directly (8-10). R-Spondin 3 has also been identified as an oncogene (11).

Product Categories/Family for anti-RSPO1 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 339276103
• NCBI GeneID: 284654
• NCBI Accession #: NP_001229839.1
• NCBI GenBank Nucleotide #: NM_001242910.1
• UniProt Accession #: Q2MKA7; Q0H8S6; Q14C72; Q5T0F2; Q8N7L5; A2A420
• Molecular Weight: 28,959 Da
• NCBI Official Full Name: R-spondin-1 isoform 3
• NCBI Official Synonym Full Names: R-spondin 1
• NCBI Official Symbol: RSPO1
• NCBI Official Synonym Symbols: RSPO; CRISTIN3; RP11-566C13.1
• NCBI Protein Information: R-spondin-1; R-spondin homolog; roof plate-specific spondin-1
• UniProt Protein Name: R-spondin-1
• Protein Family: R-spondin
• UniProt Gene Name: RSPO1
• UniProt Synonym Gene Names: hRspo1
• UniProt Entry Name: RSPO1_HUMAN

NCBI Description

This gene is a member of the R-spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. [provided by RefSeq, Jul 2008]

Uniprot Description

R-spondin-1: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Secreted; Secreted, signal peptide; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: extracellular space; extracellular region; nucleus

Molecular Function: heparin binding; protein binding; G-protein-coupled receptor binding; receptor binding

Biological Process: regulation of gene expression; regulation of receptor internalization; positive regulation of protein amino acid phosphorylation; Wnt receptor signaling pathway through beta-catenin; male meiosis; positive regulation of Wnt receptor signaling pathway

Disease: Palmoplantar Hyperkeratosis With Squamous Cell Carcinoma Of Skin And 46,xx Sex Reversal