Western Blot (WB)
(Western blot analysis of RRP8 expression in Hela (A), MCF7 (B), Hela (C) whole cell lysates.)
Rabbit anti-Human RRP8 Polyclonal Antibody | anti-RRP8 antibody
Anti-RRP8 Antibody
Western Blot (WB)
(Western blot analysis of RRP8 expression in Hela (A), MCF7 (B), Hela (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of RRP8 expression in Hela (A), MCF7 (B), Hela (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of RRP8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of RRP8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
Uniprot Description
RRP8: Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys- 9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown. Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8. Belongs to the methyltransferase superfamily. RRP8 family.
Protein type: RNA-binding; EC 2.1.1.-; Nucleolus
Chromosomal Location of Human Ortholog: 11p15.4
Cellular Component: chromatin silencing complex; cytoplasm; nucleolus; nucleoplasm; nucleus; plasma membrane
Molecular Function: methylated histone residue binding; protein binding; S-adenosylmethionine-dependent methyltransferase activity
Biological Process: cellular response to glucose starvation; chromatin modification; chromatin silencing at rDNA; gene expression; methylation; negative regulation of gene expression, epigenetic; regulation of gene expression, epigenetic; regulation of transcription by glucose; rRNA processing; transcription, DNA-dependent
Research Articles on RRP8
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Product Notes
The RRP8 rrp8 (Catalog #AAA8242425) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RRP8 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RRP8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the RRP8 rrp8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RRP8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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