Western Blot (WB)
(KIAA0409 polyclonal antibody. Western Blot analysis of KIAA0409 expression in HeLa.)
Mouse anti-Human KIAA0409 Polyclonal Antibody | anti-RRP8 antibody
KIAA0409 (Ribosomal RNA-processing Protein 8, Cerebral Protein 1, Nucleomethylin, KIAA0409, NML, hucep-1)
Gene Names
RRP8; KIAA0409
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
KIAA0409; Polyclonal Antibody; KIAA0409 (Ribosomal RNA-processing Protein 8; Cerebral Protein 1; Nucleomethylin; NML; hucep-1); Anti -KIAA0409 (Ribosomal RNA-processing Protein 8; anti-RRP8 antibody
Host
Mouse
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human KIAA0409.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MFEEPEWAEAAPVAAGLGPVISRPPPAASSQNKGSKRRQLLATLRALEAASLSQHPPSLCISDSEEEEEERKKKCPKKASFASASAEVGKKGKKKCQKQGPPCSDSEEEVERKKKCHKQALVGSDSAEDEKRKRKCQKHAPINSAQHLDNVDQTGPKAWKGSTTNDPPKQSPGSTSPKPPHTLSRKQWRNRQKNKRRCKNKFQPPQVPDQAPAEAPTEKTEVSPVPRTDSHEARAGALRARMAQRLDGARFRYLNEQLYSGPSSAAQRLFQEDPEAFLLYHRGFQSQVKKWPLQPVDRIARDLRQRPASLVVADFGCGDCRLASSIRNPVHCFDLASLDPRVTVCDMAQVPLEDESVDVAVFCLSLMGTNIRDFLEEANRVLKPGGLLKVAEVSSRFEDVRTFLRAVTKLGFKIVSKDLTNSHFFLFDFQKTGPPLVGPKAQLSGLQLQPCLYKRR
Applicable Applications for anti-RRP8 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Immunogen
Full length human KIAA0409, aa1-456 (NP_056139.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(KIAA0409 polyclonal antibody. Western Blot analysis of KIAA0409 expression in HeLa.)
Western Blot (WB)
(KIAA0409 polyclonal antibody. Western Blot analysis of KIAA0409 expression in HeLa.)
Western Blot (WB)
(Western Blot analysis of KIAA0409 expression in transfected 293T cell line by KIAA0409 polyclonal antibody. Lane 1: KIAA0409 transfected lysate (50.16kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of KIAA0409 expression in transfected 293T cell line by KIAA0409 polyclonal antibody. Lane 1: KIAA0409 transfected lysate (50.16kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-RRP8 antibody
Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.
Product Categories/Family for anti-RRP8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
50,715 Da
NCBI Official Full Name
KIAA0409
NCBI Official Synonym Full Names
ribosomal RNA processing 8, methyltransferase, homolog (yeast)
NCBI Official Symbol
RRP8
NCBI Official Synonym Symbols
KIAA0409
NCBI Protein Information
ribosomal RNA-processing protein 8; nucleomethylin; cerebral protein 1; RRP8 methyltransferase homolog
UniProt Protein Name
Ribosomal RNA-processing protein 8
Protein Family
UniProt Gene Name
RRP8
UniProt Synonym Gene Names
KIAA0409; NML
UniProt Entry Name
RRP8_HUMAN
Uniprot Description
Function: Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown. Ref.11
Subunit structure: Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8. Ref.11
Subcellular location: Nucleus › nucleolus. Note: Localizes at rDNA locus. Ref.4 Ref.5 Ref.11
Sequence similarities: Belongs to the methyltransferase superfamily. RRP8 family.
Sequence caution: The sequence BAA23705.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on RRP8
Similar Products
Product Notes
The RRP8 rrp8 (Catalog #AAA649857) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KIAA0409 (Ribosomal RNA-processing Protein 8, Cerebral Protein 1, Nucleomethylin, KIAA0409, NML, hucep-1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KIAA0409 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the RRP8 rrp8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MFEEPEWAEA APVAAGLGPV ISRPPPAASS QNKGSKRRQL LATLRALEAA SLSQHPPSLC ISDSEEEEEE RKKKCPKKAS FASASAEVGK KGKKKCQKQG PPCSDSEEEV ERKKKCHKQA LVGSDSAEDE KRKRKCQKHA PINSAQHLDN VDQTGPKAWK GSTTNDPPKQ SPGSTSPKPP HTLSRKQWRN RQKNKRRCKN KFQPPQVPDQ APAEAPTEKT EVSPVPRTDS HEARAGALRA RMAQRLDGAR FRYLNEQLYS GPSSAAQRLF QEDPEAFLLY HRGFQSQVKK WPLQPVDRIA RDLRQRPASL VVADFGCGDC RLASSIRNPV HCFDLASLDP RVTVCDMAQV PLEDESVDVA VFCLSLMGTN IRDFLEEANR VLKPGGLLKV AEVSSRFEDV RTFLRAVTKL GFKIVSKDLT NSHFFLFDFQ KTGPPLVGPK AQLSGLQLQP CLYKRR. It is sometimes possible for the material contained within the vial of "KIAA0409, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
