Rabbit RRP8 Polyclonal Antibody | anti-RRP8 antibody

RRP8 Rabbit Polyclonal

Cat. Num. AAA7603734

• Gene Names: RRP8; NML; KIAA0409
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: RRP8; Polyclonal Antibody; RRP8 Rabbit Polyclonal; anti-RRP8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 456

• Applicable Applications for anti-RRP8 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Immunogen: Ribosomal RNA processing 8, methyltransferase, homolog (yeast)
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human skin using MBS7603734 (RRP8 antibody) at dilution of 1:100)

Western Blot (WB)

(human brain tissue were subjected to SDS PAGE followed by western blot with MBS7603734 (RRP8 antibody) at dilution of 1:1500)

NCBI and Uniprot Product Information

• NCBI GI #: 12758125
• NCBI GeneID: 23378
• NCBI Accession #: NP_056139.1
• NCBI GenBank Nucleotide #: NM_015324.3
• Molecular Weight: 50,715 Da
• NCBI Official Full Name: ribosomal RNA-processing protein 8
• NCBI Official Synonym Full Names: ribosomal RNA processing 8, methyltransferase, homolog (yeast)
• NCBI Official Symbol: RRP8
• NCBI Official Synonym Symbols: NML; KIAA0409
• NCBI Protein Information: ribosomal RNA-processing protein 8
• UniProt Protein Name: Ribosomal RNA-processing protein 8
• Protein Family: 25S rRNA
• UniProt Gene Name: RRP8
• UniProt Synonym Gene Names: KIAA0409; NML
• UniProt Entry Name: RRP8_HUMAN

Uniprot Description

RRP8: Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys- 9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown. Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8. Belongs to the methyltransferase superfamily. RRP8 family.

Protein type: Nucleolus; RNA-binding; EC 2.1.1.-

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: chromatin silencing complex; cytoplasm; nucleolus; nucleoplasm; nucleus; plasma membrane

Molecular Function: methylated histone residue binding; protein binding; S-adenosylmethionine-dependent methyltransferase activity

Biological Process: cellular response to glucose starvation; chromatin modification; chromatin silencing at rDNA; methylation; regulation of transcription by glucose; rRNA processing; transcription, DNA-dependent