Rabbit anti-Human, Mouse RRP4 Polyclonal Antibody | anti-EXOSC2 antibody

RRP4 Antibody

Cat. Num. AAA9629218

• Gene Names: EXOSC2; p7; RRP4; Rrp4p; hRrp4p
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: RRP4; Polyclonal Antibody; RRP4 Antibody; EXOSC2; Exosome complex exonuclease RRP4; Exosome component 2; Homolog of yeast RRP4 (ribosomal RNA processing 4); 3' 5' exoribonuclease (RRP4); 3'-5'-exoribonuclease; hRrp4p; OTTHUMP00000022370; OTTHUMP00000022371; OTTHUMP00000022372; OTTHUMP00000022373; OTTHUMP00000022374; p7; Ribosomal RNA processing protein 4; Rrp4p; anti-EXOSC2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: RRP4 Antibody detects endogenous levels of total RRP4.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-EXOSC2 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: Belongs to the RRP4 family.
• Subunit Structure: Component of the RNA exosome complex. Specifically part of the catalytically inactive RNA exosome core (Exo-9) complex which is believed to associate with catalytic subunits EXOSC10, and DIS3 or DIS3L in cytoplasmic- and nuclear-specific RNA exosome complex forms. Exo-9 is formed by a hexameric ring of RNase PH domain-containing subunits specifically containing the heterodimers EXOSC4-EXOSC9, EXOSC5-EXOSC8 and EXOSC6-EXOSC7, and peripheral S1 domain-containing components EXOSC1, EXOSC2 and EXOSC3 located on the top of the ring structure. Interacts with DIS3. Interacts with GTPBP1. Interacts with ZFP36L1 (via N-terminus).
• Function: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC2 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC4 and EXOSC7.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-EXOSC2 antibody

Cytoplasm; Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 13878748
• NCBI GeneID: 23404
• UniProt Accession #: Q13868; Q9NUY4; A3KFL3; B4DKK6
• Molecular Weight: 293
• NCBI Official Full Name: Exosome complex component RRP4
• NCBI Official Synonym Full Names: exosome component 2
• NCBI Official Symbol: EXOSC2
• NCBI Official Synonym Symbols: p7; RRP4; Rrp4p; hRrp4p
• NCBI Protein Information: exosome complex component RRP4; exosome complex exonuclease RRP4; ribosomal RNA-processing protein 4; homolog of yeast RRP4 (ribosomal RNA processing 4), 3'-5'-exoribonuclease; homolog of yeast RRP4 (ribosomal RNA processing 4), 3' 5' exoribonuclease (RRP
• UniProt Protein Name: Exosome complex component RRP4
• UniProt Gene Name: EXOSC2
• UniProt Synonym Gene Names: RRP4
• UniProt Entry Name: EXOS2_HUMAN

Uniprot Description

RRP4: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC2 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC4 and EXOSC7.

Protein type: RNA processing; EC 3.1.13.-; Ribonuclease; Nucleolus

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: cytoplasm; nucleolus; exosome (RNase complex); cytosol; nucleus; nuclear exosome (RNase complex)

Molecular Function: protein binding; 3'-5'-exoribonuclease activity; exoribonuclease activity; 7S RNA binding

Biological Process: gene expression; exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); positive regulation of cell growth; rRNA processing; mRNA catabolic process, deadenylation-dependent decay