Rabbit RRM1 Polyclonal Antibody | anti-RRM1 antibody

RRM1 Antibody

Cat. Num. AAA9403319

• Gene Names: RRM1; R1; RR1; RIR1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: RRM1; Polyclonal Antibody; RRM1 Antibody; R1; RIR1; RR1; anti-RRM1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total RRM1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 792

• Applicable Applications for anti-RRM1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human RRM1.
• Target Name: RRM1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using RRM1 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using RRM1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-RRM1 antibody

Ribonucleotide reductase catalyzes the rate-limiting step in the synthesis of deoxynucleotide triphosphates (dNTPs). The regulatory M1 subunit (RRM1) is present throughout the cell division cycle, but downregulated in quiescent cells (1). Research studies have demonstrated that RRM1 is involved in carcinogenesis and tumor progression, and its expression is correlated with resistance to chemotherapy in non-small cell lung cancer (2-4).

Product Categories/Family for anti-RRM1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4506749
• NCBI GeneID: 6240
• NCBI Accession #: NP_001024.1
• NCBI GenBank Nucleotide #: NM_001033.3
• UniProt Accession #: P23921; Q9UNN2
• Molecular Weight: 90,070 Da
• NCBI Official Full Name: ribonucleoside-diphosphate reductase large subunit
• NCBI Official Synonym Full Names: ribonucleotide reductase M1
• NCBI Official Symbol: RRM1
• NCBI Official Synonym Symbols: R1; RR1; RIR1
• NCBI Protein Information: ribonucleoside-diphosphate reductase large subunit
• UniProt Protein Name: Ribonucleoside-diphosphate reductase large subunit
• UniProt Gene Name: RRM1
• UniProt Synonym Gene Names: RR1
• UniProt Entry Name: RIR1_HUMAN

NCBI Description

This gene encodes one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region. [provided by RefSeq, Jul 2008]

Uniprot Description

RRM1: an enzyme involved in DNA replication that provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Belongs to the ribonucleoside diphosphate reductase large chain family. Heterodimer of a large and a small subunit. Heterodimer with small subunit RRM2 or RRM2B. The heterodimer with RRM2 has higher catalytic activity than the heterodimer with RRM2B. Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding to separate specificity and activation sites on the M1 subunit. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction. Stimulated by ATP and inhibited by dATP binding to the activity site. Two distinct regulatory sites have been defined: the specificity site, which controls substrate specificity, and the activity site which regulates overall catalytic activity. A substrate-binding catalytic site, located on M1, is formed only in the presence of the second subunit M2. The level of the enzyme activity is closely correlated with the growth rate of a cell and appears to vary with the cell cycle. Patients with advanced non-small cell lung cancer responded favorably to gemcitabine.

Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; DNA replication; Other Amino Acids Metabolism - glutathione; EC 1.17.4.1; Oxidoreductase

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: nucleoplasm; cell projection; cell soma; cytoplasm; nuclear envelope; cytosol

Molecular Function: protein binding; ATP binding; ribonucleoside-diphosphate reductase activity

Biological Process: pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; retina development in camera-type eye; cell proliferation in forebrain; male gonad development; protein heterotetramerization; deoxyribonucleotide biosynthetic process; response to ionizing radiation; mitotic cell cycle; DNA replication