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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Vasoactive intestinal polypeptide receptor 1 (VIPR1) Standard Curve.)

Human Vasoactive Intestinal Polypeptide Receptor 1 (VIPR1) ELISA Kit | VIPR1 elisa kit

Human Vasoactive Intestinal Polypeptide Receptor 1 (VIPR1) ELISA Kit

Gene Names
VIPR1; II; HVR1; RDC1; V1RG; VIPR; VIRG; VAPC1; VPAC1; VPAC1R; VIP-R-1; VPCAP1R; PACAP-R2; PACAP-R-2
Reactivity
Human
Synonyms
Vasoactive Intestinal Polypeptide Receptor 1 (VIPR1); Human Vasoactive Intestinal Polypeptide Receptor 1 (VIPR1) ELISA Kit; VIPR1; FLJ41949; HVR1; PACAP-R-2; RDC1; V1RG; VAPC1; VIPR; VIRG; VPAC1; VPCAP1R; PACAP type receptor; VIP receptor; type I; pituitary adenylate cyclase activating polypeptide receptor; type; VIPR1 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human Vasoactive intestinal polypeptide receptor 1 (VIPR1) ELISA Kit has high sensitivity and excellent specificity for detection of Human VIPR1. No significant cross-reactivity or interference between Human VIPR1 and analogues was observed.
Sequence Length
247
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Vasoactive intestinal polypeptide receptor 1 (VIPR1) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Vasoactive intestinal polypeptide receptor 1 (VIPR1) Standard Curve.)
Related Product Information for VIPR1 elisa kit
Description: This Human Vasoactive intestinal polypeptide receptor 1 (VIPR1) ELISA Kit employs a two-site sandwich ELISA to quantitate VIPR1 in samples. An antibody specific for VIPR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VIPR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VIPR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VIPR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Vasoactive intestinal peptide receptor 1 is expressed in the brain (cerebral cortex, hippocampus, amygdala), lung, prostate, peripheral blood leukocytes, liver, small intestine, heart, spleen, placenta, kidney, thymus and testis. This gene encodes a receptor for vasoactive intestinal peptide, a small neuropeptide. Vasoactive intestinal peptide is involved in smooth muscle relaxation, exocrine and endocrine secretion, and water and ion flux in lung and intestinal epithelia. Its actions are effected through integral membrane receptors associated with a guanine nucleotide binding protein which activates adenylate

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
vasoactive intestinal polypeptide receptor 1 isoform 3
NCBI Official Synonym Full Names
vasoactive intestinal peptide receptor 1
NCBI Official Symbol
VIPR1
NCBI Official Synonym Symbols
II; HVR1; RDC1; V1RG; VIPR; VIRG; VAPC1; VPAC1; VPAC1R; VIP-R-1; VPCAP1R; PACAP-R2; PACAP-R-2
NCBI Protein Information
vasoactive intestinal polypeptide receptor 1
UniProt Protein Name
Vasoactive intestinal polypeptide receptor 1
UniProt Gene Name
VIPR1
UniProt Synonym Gene Names
VIP-R-1; PACAP type II receptor; PACAP-R-2; PACAP-R2
UniProt Entry Name
VIPR1_HUMAN

NCBI Description

This gene encodes a receptor for vasoactive intestinal peptide, a small neuropeptide. Vasoactive intestinal peptide is involved in smooth muscle relaxation, exocrine and endocrine secretion, and water and ion flux in lung and intestinal epithelia. Its actions are effected through integral membrane receptors associated with a guanine nucleotide binding protein which activates adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

VIPR1: This is a receptor for VIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The affinity is VIP = PACAP-27 > PACAP-38. Belongs to the G-protein coupled receptor 2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; Motility/polarity/chemotaxis; GPCR, family 2

Chromosomal Location of Human Ortholog: 3p22

Cellular Component: integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: protein binding; vasoactive intestinal polypeptide receptor activity

Biological Process: synaptic transmission; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cyclic nucleotide second messenger; muscle contraction; positive regulation of cell proliferation; digestion; immune response

Research Articles on VIPR1

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Product Notes

The Human VIPR1 vipr1 (Catalog #AAA9713722) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713722 ELISA Kit recognizes Human VIPR1. It is sometimes possible for the material contained within the vial of "Vasoactive Intestinal Polypeptide Receptor 1 (VIPR1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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