Human Biotinidase (BTD) ELISA Kit | BTD elisa kit

Human Biotinidase (BTD) ELISA Kit

Cat. Num. AAA2706706

• Reactivity: Human
• Synonyms: Biotinidase (BTD); Human Biotinidase (BTD) ELISA Kit; Biotinase; BTD elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Biotinidase (BTD).; No significant cross-reactivity or interference between Biotinidase (BTD) and analogues was observed.
• Sequence Length: 523

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Biotinidase (BTD) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Biotinidase (BTD) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BTD elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Biotinidase (BTD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Biotinidase (BTD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Biotinidase (BTD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Biotinidase (BTD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 468824
• NCBI GeneID: 686
• Molecular Weight: 58,913 Da
• NCBI Official Full Name: biotinidase
• NCBI Official Synonym Full Names: biotinidase
• NCBI Official Symbol: BTD
• NCBI Protein Information: biotinidase
• UniProt Protein Name: Biotinidase
• Protein Family: Biotinidase
• UniProt Gene Name: BTD
• UniProt Synonym Gene Names: Biotinase

NCBI Description

The protein encoded by this gene functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013]

Uniprot Description

Catalytic release of biotin from biocytin, the product of biotin-dependent carboxylases degradation.