Rabbit anti-Human RPS6KB1 Polyclonal Antibody | anti-RPS6KB1 antibody

RPS6KB1 (Ser424) (Ribosomal Protein S6 Kinase beta-1, S6K-beta-1, S6K1, 70kD Ribosomal Protein S6 Kinase 1, P70S6K1, p70-S6K 1, p70 Ribosomal S6 Kinase alpha, p70 S6 Kinase alpha, p70 S6K-alpha, p70 S6KA, p70-S6K, Ribosomal Protein S6 Kinase I, Serine/thr

Cat. Num. AAA6497711

• Gene Names: RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: RPS6KB1; Polyclonal Antibody; RPS6KB1 (Ser424) (Ribosomal Protein S6 Kinase beta-1; S6K-beta-1; S6K1; 70kD Ribosomal Protein S6 Kinase 1; P70S6K1; p70-S6K 1; p70 Ribosomal S6 Kinase alpha; p70 S6 Kinase alpha; p70 S6K-alpha; p70 S6KA; p70-S6K; Ribosomal Protein S6 Kinase I; Serine/thr; Ribosomal Protein S6 Kinase 70kDa Polypeptide 1; EC=2.7.11.1; p70-alpha; p70(S6K)-alpha; PS6K; anti-RPS6KB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RPS6KB1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RPS6KB1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acid residues surrounding S424 of human RPS6KB1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RPS6KB1 (arrow) using rabbit polyclonal RPS6KB1 Antibody (S424) (RMBS6160734). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RPS6KB1 gene (Lane 2) (Origene Technologies).))

Western Blot (WB)

(Western blot analysis of Phospho-RPS6KB1-pS424 Antibody (AMBS600980a) and RPS6KB1 Antibody (S424) (AP3289g) in human TNF alpha activated Hela cell line lysates. RPS6KB1 and Phospho-RPS6KB1 (arrow) was detected using the purified PAb. (lane 1: without TNF alpha; lane 2: TNF alpha activated, Phospho-RPS6KB1-pS424 Antibody (AMBS600980a); lane 3: TNF alpha activated, RPS6KB1 Antibody (S424) (AP3289g))

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with RPS6KB1 Antibody (T421), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-RPS6KB1 antibody

Antibodies; Protein Kinases; Ribosomal

References

1. Adem, C., et al., Genes Chromosomes Cancer 41(1):1-11 (2004). 2. Suzuki, Y., et al., Genome Res. 14(9):1711-1718 (2004). 3. Gomez-Cambronero, J., et al., Leuk. Res. 28(7):755-762 (2004). 4. Raught, B., et al., EMBO J. 23(8):1761-1769 (2004). 5. Miyakawa, M., et al., Endocr. J. 50(1):77-83 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4506737
• NCBI GeneID: 6198
• NCBI Accession #: NP_003152
• NCBI GenBank Nucleotide #: NM_003161.3
• UniProt Accession #: P23443; Q7Z721; B2R779; B4DLT4; B4DTG1; E7ESB8; F6UYM1
• Molecular Weight: 60.2kDa (533aa) 70-100kDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: ribosomal protein S6 kinase beta-1 isoform a
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase B1
• NCBI Official Symbol: RPS6KB1
• NCBI Official Synonym Symbols: S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
• NCBI Protein Information: ribosomal protein S6 kinase beta-1
• UniProt Protein Name: Ribosomal protein S6 kinase beta-1
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: RPS6KB1
• UniProt Synonym Gene Names: STK14A; S6K-beta-1; S6K1; P70S6K1; p70-S6K 1; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA
• UniProt Entry Name: KS6B1_HUMAN

NCBI Description

This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]

Uniprot Description

p70S6K: an AGC kinase of the RSK family that is required for cell growth and G1 cell cycle progression. Is phosphorylated and activated by mTOR in mitogenic pathways downstream of phosphoinositide 3 kinase (PI3K). Phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. Activity is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains. Mouse knockout shows symptoms of insulin resistance, and increased insulin senstivity, resulting in protection against diet-induced obesity. Protein expression and activation upregulated in colon adenocarcinoma cell lines. Increased expression in breast cancer correlated with poor survival. Selectively amplified and overexpressed within the 17q23 breast cancer amplicon. Two isoforms produced by alternative initiation have been reported.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; Translation; AGC group; RSK family; p70 subfamily

Chromosomal Location of Human Ortholog: 17q23.1