Rabbit anti-Human RPS13 Polyclonal Antibody | anti-RPS13 antibody

RPS13, ID (RPS13, 40S ribosomal protein S13) (PE)

Cat. Num. AAA6343731

• Gene Names: RPS13; S13
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPS13; Polyclonal Antibody; ID (RPS13; 40S ribosomal protein S13) (PE); 40S ribosomal protein S13; anti-RPS13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RPS13 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RPS13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89~118 amino acids from the Central region of human RPS13.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RPS13 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). RPS13 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(RPS13 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPS13 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RPS13 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RPS13 antibody

RPS13, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S15P family of ribosomal proteins. It is located in the cytoplasm. The protein has been shown to bind to the 5.8S rRNA in rat. The gene product of the E. coli ortholog (ribosomal protein S15) functions at early steps in ribosome assembly. This gene is co-transcribed with two U14 small nucleolar RNA genes, which are located in its third and fifth introns. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Product Categories/Family for anti-RPS13 antibody

Antibodies; Ribosomal Proteins

References

Malygin, A.A., et al. Nucleic Acids Res. 35(19):6414-6423(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006). Yu, Y., et al. Protein Sci. 14(6):1438-1446(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 4506685
• NCBI GeneID: 6207
• NCBI Accession #: NP_001008.1
• NCBI GenBank Nucleotide #: NM_001017.2
• UniProt Accession #: P62277; P19116; Q02546; Q29200; Q498Y0; B2R549
• Molecular Weight: 17,222 Da
• NCBI Official Full Name: 40S ribosomal protein S13
• NCBI Official Synonym Full Names: ribosomal protein S13
• NCBI Official Symbol: RPS13
• NCBI Official Synonym Symbols: S13
• NCBI Protein Information: 40S ribosomal protein S13
• UniProt Protein Name: 40S ribosomal protein S13
• Protein Family: 40S ribosomal protein
• UniProt Gene Name: RPS13
• UniProt Entry Name: RS13_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S15P family of ribosomal proteins. It is located in the cytoplasm. The protein has been shown to bind to the 5.8S rRNA in rat. The gene product of the E. coli ortholog (ribosomal protein S15) functions at early steps in ribosome assembly. This gene is co-transcribed with two U14 small nucleolar RNA genes, which are located in its third and fifth introns. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPS13: a ribosomal protein of the S15P family. Located in the cytoplasm and nucleus. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 40S subunit. Binds to the 5.8S rRNA.

Protein type: Translation; Ribosomal

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: focal adhesion; membrane; nucleolus; ribosome; cytosol; nucleus

Molecular Function: mRNA binding; protein binding; structural constituent of ribosome

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; negative regulation of RNA splicing; viral reproduction; translation; mRNA catabolic process, nonsense-mediated decay; translational initiation; viral transcription; gene expression; translational termination; viral infectious cycle