ACO1 / irp1 recombinant protein

Recombinant Human ACO1 / irp1 Protein (His tag)

Cat. Num. AAA2545991

• Gene Names: ACO1; IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: ACO1 / irp1; Recombinant Human ACO1 / irp1 Protein (His tag); RP11-334P12.1; ACONS; IREB1; IREBP; IREBP1; IRP1; ACO1 / irp1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% gly, 2mM DTT
• Sequence Length: 889

• Application Notes: The recombinant human ACO1 consists of 908 amino acids and has a calculated molecular mass of 101 kDa. It migrates as an approximately 90 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ACO1 / irp1 recombinant protein

Background: Aconitase 1 (ACO1) or IRP1 is one member of the aconitase family that contains a diverse group of iron-sulphur (Fe-S) isomerases and two type s of iron regulatory protein. Aconitase exits in two forms: one is soluble and the other is mitochondrial. ACO1 is the soluble existing form, and the mitochondrial form is ACO2. Residues from all three N-terminal domains and the larger C-terminal domain contribute to the active site region. When the enzyme is activated, it gains an additional iron atom. ACO1 can assume two different functions in cells, depending on different conditions. During iron scarcity or oxidative stress, ACO1 binds to mRNA stem-loop structures called iron responsive elements to modulate the translation of iron metabolism genes. In iron-rich conditions, ACO1 binds an iron-sulfur cluster to function as a cytosolic aconitase. 

Description: A DNA sequence encoding the human ACO1 (P21399) (Met 1-Lys 889) was expressed, with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 507834063
• NCBI GeneID: 48
• NCBI Accession #: NP_001265281.1
• NCBI GenBank Nucleotide #: NM_001278352.1
• UniProt Accession #: P21399; Q14652; Q5VZA7; D3DRK7
• Molecular Weight: 98,399 Da
• NCBI Official Full Name: cytoplasmic aconitate hydratase
• NCBI Official Synonym Full Names: aconitase 1
• NCBI Official Symbol: ACO1
• NCBI Official Synonym Symbols: IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1
• NCBI Protein Information: cytoplasmic aconitate hydratase
• UniProt Protein Name: Cytoplasmic aconitate hydratase
• UniProt Gene Name: ACO1
• UniProt Synonym Gene Names: IREB1; Aconitase; IRP1; IRE-BP 1

NCBI Description

The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]

Uniprot Description

IREB1: Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding. Belongs to the aconitase/IPM isomerase family.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 4.2.1.3; Endoplasmic reticulum; Lyase; RNA-binding; Translation

Chromosomal Location of Human Ortholog: 9p21.1

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; Golgi apparatus; mitochondrion

Molecular Function: 4 iron, 4 sulfur cluster binding; aconitate hydratase activity; iron-responsive element binding; protein binding; RNA binding

Biological Process: citrate metabolic process; response to iron(II) ion