Rabbit anti-Human RPLP0 Polyclonal Antibody | anti-RPLP0 antibody

RPLP0, NT (RPLP0, 60S acidic ribosomal protein P0, 60S ribosomal protein L10E)

Cat. Num. AAA646258

• Gene Names: RPLP0; P0; LP0; L10E; RPP0; PRLP0
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RPLP0; Polyclonal Antibody; NT (RPLP0; 60S acidic ribosomal protein P0; 60S ribosomal protein L10E); Anti -RPLP0; anti-RPLP0 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-RPLP0 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: RPLP0 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RPLP0.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RPLP0 Antibody (N-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the RPLP0 antibody detected the RPLP0 protein (arrow).)

Related Product Information for anti-RPLP0 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Product Categories/Family for anti-RPLP0 antibody

Antibodies; Abs to Ribosomal Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 78070390
• NCBI GeneID: 6175
• UniProt Accession #: P05388; Q9BVK4
• Molecular Weight: 34,274 Da
• NCBI Official Full Name: RPLP0 protein
• NCBI Official Synonym Full Names: ribosomal protein, large, P0
• NCBI Official Symbol: RPLP0
• NCBI Official Synonym Symbols: P0; LP0; L10E; RPP0; PRLP0
• NCBI Protein Information: 60S acidic ribosomal protein P0; 60S ribosomal protein L10E; acidic ribosomal phosphoprotein P0
• UniProt Protein Name: 60S acidic ribosomal protein P0
• UniProt Gene Name: RPLP0
• UniProt Entry Name: RLA0_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Ribosomal protein P0 is the functional equivalent of E.coli protein L10.

Subunit structure: P0 forms a pentameric complex by interaction with dimers of P1 and P2. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with Lassa virus Z protein. Interacts with APEX1. Ref.6 Ref.7

Subcellular location: Nucleus. Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Ref.6 Ref.7

Sequence similarities: Belongs to the ribosomal protein L10P family.