Rabbit anti-Human RPL9 Polyclonal Antibody | anti-RPL9 antibody

Anti-RPL9 Antibody, Rabbit Polyclonal

Cat. Num. AAA8108818

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: RPL9; Polyclonal Antibody; Anti-RPL9 Antibody; Rabbit Polyclonal; RPL9 Antibody; Rabbit PAb; Antigen Affinity Purified; ribosomal protein L9; Anti-L9 Antibody; Anti-NPC-A-16 Antibody; anti-RPL9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human RPL9
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-RPL9 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:50-1:200; ICC/IF: 1:100-1:500; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human RPL9 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human RPL9 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-RPL9 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: SH-SY5Y Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:22 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human RPL9 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human RPL9 in human pancreas with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of RPL9 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human RPL9 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Immunoprecipitation (IP)

(RPL9 was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-RPL9 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RPL9 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 22 kDa Observed band size :22 kDa)

Related Product Information for anti-RPL9 antibody

RPL9 (Ribosomal Protein L9) is a Protein Coding gene. The RPL9 gene, located on 4p14, is conserved in chimpanzee, Rhesus monkey, dog, mouse, rat, and chicken, etc. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L6P family of ribosomal proteins. It is located in the cytoplasm. RPL9, a component of the large subunit of the ribosome, has an unusual structure, comprising two compact globular domains connected by an alpha-helix; it interacts with 23 S rRNA. Diseases associated with RPL9 include Combined Oxidative Phosphorylation Deficiency 1 and Diamond-Blackfan Anemia. Among its related pathways are rRNA processing in the nucleus and cytosol and Viral mRNA Translation.

References

Hou WR, et al. (2011) Cdna, genomic sequence cloning and overexpression of ribosomal protein gene l9 (rpl9) of the giant panda (ailuropoda melanoleuca). Genet Mol Res 10 (3): 1576-1588.Eid R, et al. (2014) Human ribosomal protein l9 is a bax suppressor that promotes cell survival in yeast. FEMS Yeast Res 14 (3): 495-507.Baik IH, et al. (2016) Knockdown of rpl9 expression inhibits colorectal carcinoma growth via the inactivation of id-1/nf-kappab signaling axis. Int J Oncol 49 (5): 1953-1962.