Rabbit anti-Human RPL7 Polyclonal Antibody | anti-RPL7 antibody

RPL7, NT (RPL7, 60S ribosomal protein L7) (Azide free) (HRP)

Cat. Num. AAA6343582

• Gene Names: RPL7; L7; humL7-1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPL7; Polyclonal Antibody; NT (RPL7; 60S ribosomal protein L7) (Azide free) (HRP); 60S ribosomal protein L7; anti-RPL7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RPL7 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RPL7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-35 amino acids from the N-terminal region of human RPL7.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RPL7 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the RPL7 antibody detected the RPL7 protein (arrow).)

Related Product Information for anti-RPL7 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30P family of ribosomal proteins. It contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus submotif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus. It is located in the cytoplasm. The protein has been shown to be an autoantigen in patients with systemic autoimmune diseases, such as systemic lupus erythematosus. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Product Categories/Family for anti-RPL7 antibody

Antibodies; Ribosomal Proteins

References

Chou, C.W., et al. FEBS Lett. 584(19):4151-4156(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Maggi, L.B. Jr., et al. Mol. Cell. Biol. 28(23):7050-7065(2008). Robledo, S., et al. RNA 14(9):1918-1929(2008). Wu, W.C., et al. FEBS Lett. 581(4):651-657(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 15431301
• NCBI GeneID: 6129
• NCBI Accession #: NP_000962.2
• NCBI GenBank Nucleotide #: NM_000971.3
• UniProt Accession #: P18124; Q15289; Q3KQU0; Q5I0X1; Q6IBM9; A8K504
• Molecular Weight: 29,226 Da
• NCBI Official Full Name: 60S ribosomal protein L7
• NCBI Official Synonym Full Names: ribosomal protein L7
• NCBI Official Symbol: RPL7
• NCBI Official Synonym Symbols: L7; humL7-1
• NCBI Protein Information: 60S ribosomal protein L7
• UniProt Protein Name: 60S ribosomal protein L7
• Protein Family: 60S ribosomal protein
• UniProt Gene Name: RPL7
• UniProt Entry Name: RL7_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30P family of ribosomal proteins. It contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus submotif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus. It is located in the cytoplasm. The protein has been shown to be an autoantigen in patients with systemic autoimmune diseases, such as systemic lupus erythematosus. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPL7: a ribosomal protein of the L30P family. Located in the cytoplasm. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit. Contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus submotif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. Can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus.

Protein type: RNA processing; Nuclear receptor co-regulator; Ribosomal

Chromosomal Location of Human Ortholog: 8q21.11

Cellular Component: focal adhesion; membrane; cytoplasm; nucleolus; cytosol; ribonucleoprotein complex; nucleus

Molecular Function: mRNA binding; protein binding; protein homodimerization activity; structural constituent of ribosome; DNA binding; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; translation; viral reproduction; translational termination; ribosomal large subunit biogenesis and assembly; viral infectious cycle; cellular protein metabolic process; translational elongation; mRNA catabolic process, nonsense-mediated decay; translational initiation; viral transcription; gene expression; rRNA processing